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|Title:||A striking common O-linked N-acetylglucosaminyl moiety between cruzipain and myosin||Authors:||Acosta, D. M.
Soprano, L. L.
Esteva, Mónica Inés
Couto, A. S.
Duschak, V. G.
|Issue Date:||2011||Description:||Single units of O-linked N-acetylglucosamine (GlcNAc), usually components of nuclear and cytoplasmatic proteins, are present at the C-terminal domain of cruzipain (Cz), a lysosomal major antigen from Trypanosoma cruzi. On the other hand, antibodies directed against some self-antigens like myosin are associated with Chagas heart disease. The participation of O-GlcNAc moieties in the molecular antigenicity of Cz was determined using GlcNAc linked to aprotinin by ELISA. The immune cross-reactivity between Cz and myosin is mainly focused in the C-T domain. ELISA inhibition assays using rabbit sera specific for Cz and C-T in conjunction with immune-gold electron microscopy analysis of heart tissues from mice immunized with C-T confronted with polyclonal rabbit sera specific for Cz and C-T prior and after myosin adsorption provided evidence which indicates that O-GlcNAc moieties constitute a common epitope between Cz and either myosin or other cardiac O-GlcNAc-containing proteins, showing a new insight into the molecular immune pathogenesis of Chagas heart disease.||URI:||http://sgc.anlis.gob.ar/handle/123456789/534||Rights:||info:eu-repo/semantics/openAccess|
|Appears in Collections:||Publicaciones INP|
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|Parasiteimmunology,2011,33(7),363-370..pdf||668.58 kB||Adobe PDF|
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