Please use this identifier to cite or link to this item:
|Title:||Characterisation of a novel interspersed Toxoplasma gondii DNA repeat with potential uses for PCR diagnosis and PCR-RFLP analysis||Authors:||Echeverria, Pablo C.
Rojas, Paola A.
Angel, Sergio O.
|Issue Date:||2000||Description:||A novel Toxoplasma gondii interspersed repeat element (TgIRE), present in most of the tachyzoite chromosomes, was characterised. Two regions on the TgIRE sequence showed high identity to two different T. gondii expressed sequence tag cDNAs of unknown function, which seems to be TgIRE pseudogenes. Two set of primers were designed, 2-2P and 2-3, that amplify products of 1.02 and 0.62 kb, respectively. T. gondii DNA from RH and Me49 strains was amplified with TgIRE 2-2P primers, and the respective 1.02 kb products were digested with several endonucleases. Different fragment patterns by gel electrophoresis were found only with MboI. Sensitivity analysis revealed that the set 2-3 was more sensitive than 2-2P, detecting by gel visualisation the amount of DNA equivalent to 1 and 10 parasites, respectively.||URI:||http://sgc.anlis.gob.ar/handle/123456789/496||Rights:||restrictedAccess|
|Appears in Collections:||Publicaciones INP|
Show full item record
Files in This Item:
|FEMSMicrobiologyLetters,2000,184(1),23-27..pdf||252.56 kB||Adobe PDF|
checked on Sep 21, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.