Use este identificador para citar ou linkar para este item:
http://sgc.anlis.gob.ar/handle/123456789/495
Título: | The tyrosine aminotransferase from Trypanosoma rangeli: sequence and genomic characterization | Autor(es): | Bontempi, Esteban Garcia, G. A. Buschiazzo, A. Henriksson, J. Pravia, Carlos Ruiz, Andrés Mariano Pettersson, Ulf Pszenny, Viviana |
Palavras-chave: | Trypanosoma rangeli | Data do documento: | 15-Ago-2000 | Editora: | Federation of European Microbiological Societies | Jornal: | FEMS microbiology letters | Resumo: | The complete sequence and genomic characterization of the tyrosine aminotransferase (TAT) gene from Trypanosoma rangeli is reported. The gene was found to be organized in a tandem multicopy gene array. A homologous mRNA species (2.5 kb) was identified in the epimastigote form of the parasite. From the deduced amino acid sequence, the gene encodes a protein of 420 amino acids with a predicted molecular mass of 46.4 kDa and a theoretical pI of 6.23. A high sequence identity was found with the Trypanosoma cruzi, human and rat enzymes. All the essential residues for TAT enzymatic activity are conserved, as well as a pyridoxal-phosphate attachment site typical of class-I aminotransferases. The recombinant enzyme was recognized by a monoclonal antibody against the T. cruzi enzyme. Additionally, the recombinant protein showed enzymatic activity when incubated with L-tyrosine and 2-oxoglutaric acid as substrates. |
Descrição: | Fil: Bontempi, Esteban. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina. Fil: Garcia, G. A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina. Fil: Buschiazzo, A. Instituto de Investigaciones Biotecnológicas, Universidad Nacional de Gral. San Martín, INTI; Argentina. Fil: Henriksson, J. Department of Medical Genetics and Pathology, Uppsala University, Suecia. Fil: Pravia, Carlos. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina. Fil: Ruiz, Andrés Mariano. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina. Fil: Pettersson, Ulf. Department of Medical Genetics and Pathology, Uppsala University; Suecia. Fil: Pszenny, Viviana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina. |
URI: | http://sgc.anlis.gob.ar/handle/123456789/495 | ISSN: | 1574-6968 | DOI: | 10.1111_j.1574-6968.2000.tb09239.x. | Direitos: | Open Access |
Aparece nas Coleções: | Preproducción Publicaciones INP |
Arquivos neste item:
Arquivo | Descrição | Tamanho | Formato | |
---|---|---|---|---|
FemsMicrobiologyLetters,2000,189(2),253-257..pdf | Artículo en inglés | 1.06 MB | Adobe PDF | Ver/Aberto |
Visualização de página
60
Checado em 26/04/2024
Download(s)
11
Checado em 26/04/2024
Google ScholarTM
Checar
Altmetric
Altmetric
Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.