Please use this identifier to cite or link to this item: http://sgc.anlis.gob.ar/handle/123456789/495
Title: The tyrosine aminotransferase from Trypanosoma rangeli: sequence and genomic characterization
Authors: Bontempi, Esteban 
Garcia, G. A. 
Buschiazzo, A. 
Henriksson, J. 
Pravia, Carlos 
Ruiz, Andrés Mariano 
Pettersson, Ulf 
Pszenny, Viviana 
Keywords: Trypanosoma rangeli
Issue Date: 15-Aug-2000
Publisher: Federation of European Microbiological Societies
Journal: FEMS microbiology letters 
Abstract: 
The complete sequence and genomic characterization of the tyrosine aminotransferase (TAT) gene from Trypanosoma rangeli is reported. The gene was found to be organized in a tandem multicopy gene array. A homologous mRNA species (2.5 kb) was identified in the epimastigote form of the parasite. From the deduced amino acid sequence, the gene encodes a protein of 420 amino acids with a predicted molecular mass of 46.4 kDa and a theoretical pI of 6.23. A high sequence identity was found with the Trypanosoma cruzi, human and rat enzymes. All the essential residues for TAT enzymatic activity are conserved, as well as a pyridoxal-phosphate attachment site typical of class-I aminotransferases. The recombinant enzyme was recognized by a monoclonal antibody against the T. cruzi enzyme. Additionally, the recombinant protein showed enzymatic activity when incubated with L-tyrosine and 2-oxoglutaric acid as substrates.
Description: 
Fil: Bontempi, Esteban. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.

Fil: Garcia, G. A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.

Fil: Buschiazzo, A. Instituto de Investigaciones Biotecnológicas, Universidad Nacional de Gral. San Martín, INTI; Argentina.

Fil: Henriksson, J. Department of Medical Genetics and Pathology, Uppsala University, Suecia.

Fil: Pravia, Carlos. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.

Fil: Ruiz, Andrés Mariano. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.

Fil: Pettersson, Ulf. Department of Medical Genetics and Pathology, Uppsala University; Suecia.

Fil: Pszenny, Viviana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.
URI: http://sgc.anlis.gob.ar/handle/123456789/495
ISSN: 1574-6968
DOI: 10.1111_j.1574-6968.2000.tb09239.x.
Rights: Open Access
Appears in Collections:Preproducción
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