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Title: | The tyrosine aminotransferase from Trypanosoma rangeli: sequence and genomic characterization | Authors: | Bontempi, Esteban Garcia, G. A. Buschiazzo, A. Henriksson, J. Pravia, Carlos Ruiz, Andrés Mariano Pettersson, Ulf Pszenny, Viviana |
Keywords: | Trypanosoma rangeli | Issue Date: | 15-Aug-2000 | Publisher: | Federation of European Microbiological Societies | Journal: | FEMS microbiology letters | Abstract: | The complete sequence and genomic characterization of the tyrosine aminotransferase (TAT) gene from Trypanosoma rangeli is reported. The gene was found to be organized in a tandem multicopy gene array. A homologous mRNA species (2.5 kb) was identified in the epimastigote form of the parasite. From the deduced amino acid sequence, the gene encodes a protein of 420 amino acids with a predicted molecular mass of 46.4 kDa and a theoretical pI of 6.23. A high sequence identity was found with the Trypanosoma cruzi, human and rat enzymes. All the essential residues for TAT enzymatic activity are conserved, as well as a pyridoxal-phosphate attachment site typical of class-I aminotransferases. The recombinant enzyme was recognized by a monoclonal antibody against the T. cruzi enzyme. Additionally, the recombinant protein showed enzymatic activity when incubated with L-tyrosine and 2-oxoglutaric acid as substrates. |
Description: | Fil: Bontempi, Esteban. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina. Fil: Garcia, G. A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina. Fil: Buschiazzo, A. Instituto de Investigaciones Biotecnológicas, Universidad Nacional de Gral. San Martín, INTI; Argentina. Fil: Henriksson, J. Department of Medical Genetics and Pathology, Uppsala University, Suecia. Fil: Pravia, Carlos. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina. Fil: Ruiz, Andrés Mariano. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina. Fil: Pettersson, Ulf. Department of Medical Genetics and Pathology, Uppsala University; Suecia. Fil: Pszenny, Viviana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina. |
URI: | http://sgc.anlis.gob.ar/handle/123456789/495 | ISSN: | 1574-6968 | DOI: | 10.1111_j.1574-6968.2000.tb09239.x. | Rights: | Open Access |
Appears in Collections: | Preproducción Publicaciones INP |
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File | Description | Size | Format | |
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FemsMicrobiologyLetters,2000,189(2),253-257..pdf | Artículo en inglés | 1.06 MB | Adobe PDF | View/Open |
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