Please use this identifier to cite or link to this item:
http://sgc.anlis.gob.ar/handle/123456789/495
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Bontempi, Esteban | es |
dc.contributor.author | Garcia, G. A. | es |
dc.contributor.author | Buschiazzo, A. | es |
dc.contributor.author | Henriksson, J. | es |
dc.contributor.author | Pravia, Carlos | es |
dc.contributor.author | Ruiz, Andrés Mariano | es |
dc.contributor.author | Pettersson, Ulf | es |
dc.contributor.author | Pszenny, Viviana | es |
dc.date.accessioned | 2013-05-22T17:04:27Z | - |
dc.date.available | 2013-05-22T17:04:27Z | - |
dc.date.issued | 2000-08-15 | - |
dc.identifier.issn | 1574-6968 | - |
dc.identifier.uri | http://sgc.anlis.gob.ar/handle/123456789/495 | - |
dc.description | Fil: Bontempi, Esteban. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina. | es |
dc.description | Fil: Garcia, G. A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina. | es |
dc.description | Fil: Buschiazzo, A. Instituto de Investigaciones Biotecnológicas, Universidad Nacional de Gral. San Martín, INTI; Argentina. | es |
dc.description | Fil: Henriksson, J. Department of Medical Genetics and Pathology, Uppsala University, Suecia. | es |
dc.description | Fil: Pravia, Carlos. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina. | es |
dc.description | Fil: Ruiz, Andrés Mariano. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina. | es |
dc.description | Fil: Pettersson, Ulf. Department of Medical Genetics and Pathology, Uppsala University; Suecia. | es |
dc.description | Fil: Pszenny, Viviana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina. | es |
dc.description.abstract | The complete sequence and genomic characterization of the tyrosine aminotransferase (TAT) gene from Trypanosoma rangeli is reported. The gene was found to be organized in a tandem multicopy gene array. A homologous mRNA species (2.5 kb) was identified in the epimastigote form of the parasite. From the deduced amino acid sequence, the gene encodes a protein of 420 amino acids with a predicted molecular mass of 46.4 kDa and a theoretical pI of 6.23. A high sequence identity was found with the Trypanosoma cruzi, human and rat enzymes. All the essential residues for TAT enzymatic activity are conserved, as well as a pyridoxal-phosphate attachment site typical of class-I aminotransferases. The recombinant enzyme was recognized by a monoclonal antibody against the T. cruzi enzyme. Additionally, the recombinant protein showed enzymatic activity when incubated with L-tyrosine and 2-oxoglutaric acid as substrates. | es |
dc.language.iso | en | es |
dc.publisher | Federation of European Microbiological Societies | es |
dc.relation.ispartof | FEMS microbiology letters | es |
dc.rights | Open Access | en_US |
dc.source | Fems Microbiology Letters, 2000, 189(2), 253-257. | en_US |
dc.subject | Trypanosoma rangeli | es |
dc.title | The tyrosine aminotransferase from Trypanosoma rangeli: sequence and genomic characterization | es |
dc.type | Artículo | es |
dc.identifier.doi | 10.1111_j.1574-6968.2000.tb09239.x. | - |
anlis.essnrd | 1 | - |
item.openairetype | Artículo | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
crisitem.author.dept | Instituto Nacional de Parasitología (INP) | - |
crisitem.author.dept | Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS) | - |
crisitem.author.parentorg | Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS) | - |
Appears in Collections: | Preproducción Publicaciones INP |
Files in This Item:
File | Description | Size | Format | |
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FemsMicrobiologyLetters,2000,189(2),253-257..pdf | Artículo en inglés | 1.06 MB | Adobe PDF | View/Open |
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