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Título : First insights into the biochemical and toxicological characterization of venom from the Banded Cat-eyed Snake Leptodeira annulata pulchriceps
Autor : Sánchez, Matías N 
Gonzalez, Karen 
Sciani, Juliana M 
Gritti, Micaela Andrea 
Maruñak, Silvana L 
Tavares, Flávio L 
Teibler, Gladys P 
Peichoto, María Elisa 
Palabras clave : Argentina;Toxinas;Colubridae;Enzimas;Hemorragia
Fecha de publicación : 17-sep-2020
Journal: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 
Resumen : 
With the aim to widen the current knowledge of toxinological implications of bites from rear-fanged snakes and biological roles of their venoms, this study focuses on the biochemical composition and toxic effects of the venom of Leptodeira annulata pulchriceps from Argentina. We analyzed the protein composition by electrophoresis and mass spectrometry, and enzymatic properties by quantitative assays on different substrates. Additionally, we evaluated local and systemic toxicity in mice, and tested its cross-reactivity with elapid and viperid antivenoms used in Argentina. This venom showed features reminiscent of venoms from snakes of Bothrops genus, containing components ranging from ~17 to 75 kDa, which are mainly tissue-damaging toxins such as proteinases. Although showing low lethality to mice (LD50 = 20 μg/g body weight), prominent hemorrhage developed locally in mice intramuscularly and intradermally injected with the venom, and the minimum hemorrhagic dose was found to be 12.7 μg/mouse. This study is the first comprehensive investigation of the venom of L. a. pulchriceps, and sheds new light on differences between this and those of the other two subspecies of L. annulata. Additionally, the study provides new insights into the venom components of "colubrid" snakes, advocating for considering bites from this rich diversity of snakes as a public health problem that needs to be addressed worldwide.
Descripción : 
Fil: Sánchez, Matías N. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Medicina Tropical (INMeT); Puerto Iguazú, Argentina.

Fil: Gonzalez, Karen. Universidad Nacional del Nordeste (UNNE). Facultad de Ciencias Veterinarias (FCV); Corrientes, Argentina.

Fil: Sciani, Juliana M. Universidade São Francisco. Laboratório Multidisciplinar de Pesquisa; San Pablo, Brasil.

Fil: Gritti, Micaela Andrea. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Medicina Tropical (INMeT); Puerto Iguazú, Argentina.

Fil: Maruñak, Silvana L. Universidad Nacional del Nordeste (UNNE). Facultad de Ciencias Veterinarias (FCV); Corrientes, Argentina.

Fil: Tavares, Flávio L. Universidade Federal da Integração Latino-Americana (UNILA); Foz do Iguaçu, Brasil.

Fil: Teibler, Gladys P. Universidad Nacional del Nordeste (UNNE). Facultad de Ciencias Veterinarias (FCV); Corrientes, Argentina.

Fil: Peichoto, María Elisa. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Medicina Tropical (INMeT); Puerto Iguazú, Argentina.
URI : http://sgc.anlis.gob.ar/handle/123456789/1661
ISSN : 1532-0456
DOI: 10.1016/j.cbpc.2020.108897
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