Please use this identifier to cite or link to this item:
http://sgc.anlis.gob.ar/handle/123456789/1480
Title: | Structural and immunological characterization of sulphatides: relevance of sulphate moieties in Trypanosoma cruzi glycoconjugates | Authors: | Acosta, Diana M Soprano, Luciana L Ferrero, Maximiliano R Esteva, Monica I Riarte, Adelina Couto, Alicia S Duschak, Vilma G |
Issue Date: | Nov-2012 | Journal: | Parasite immunology | Abstract: | Sulphoglycosphingolipids, present on the surface of diverse cells, participate in the regulation of various cellular events. However, little is known about the structure and the role of sulphoglycosphingolipids in trypanosomatids. Herein, sulphated dihexosylceramide structures - composed mainly of sphingosine as the long chain base acylated with stearic acid - have been determined for the first time in Trypanosoma cruzi epimastigotes by UV-MALDI-TOF-MS analysis. Interestingly, inhibition ELISA assays using cruzipain as antigen and polyclonal rabbit antibodies specific for cruzipain, the major cysteine proteinase of T. cruzi, or for its C-terminal domain, have demonstrated (i) that sulphate epitopes are shared between cruzipain and sulphatides of T. cruzi, (ii) that cross-reactivity maps to the C-terminal domain and (iii) the existence of other antigenic determinants in the glycolipidic structures. These features provide evidence that sulphate groups are antigenic in sulphate-containing parasite glycoconjugates. Furthermore, IgG2 antibody levels inversely correlate with disease severity in chronic Chagas disease patients, suggesting that IgG2 antibodies specific for sulphated epitopes might be associated with protective immunity and might be considered as potential surrogates of the course of chronic Chagas disease. |
URI: | http://sgc.anlis.gob.ar/handle/123456789/1480 | DOI: | 10.1111/j.1365-3024.2012.01378.x |
Appears in Collections: | Publicaciones INP |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
j.1365-3024.2012.01378.x.pdf | Artículo en inglés | 655.62 kB | Adobe PDF | View/Open |
Page view(s)
53
checked on Oct 16, 2024
Download(s)
9
checked on Oct 16, 2024
Google ScholarTM
Check
Altmetric
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.