Please use this identifier to cite or link to this item: http://sgc.anlis.gob.ar/handle/123456789/392
Title: Detection of human Toxoplasma-specific immunoglobulins A, M, and G with a recombinant Toxoplasma gondii rop2 protein
Authors: Martin, Valentina 
Arcavi, Miriam 
Santillan, Graciela 
Amendoeira, María Regina R. 
De Souza Neves, Elizabeth 
Griemberg, Gloria 
Guarnera, Eduardo 
Garberi, Juan C. 
Angel, Sergio O. 
Keywords: Toxoplasmosis;Humanos;Inmunoglobulina A;Inmunoglobulina M;Inmunoglobulina G
Issue Date: 1998
Description: 
The Toxoplasma gondii rhoptry protein Rop2 was expressed in Escherichia coli as a fusion protein containing 44 kDa of the 55-kDa mature Rop2, supplied with six histidyl residues at the N-terminal end (Rop2196-561). Humoral response during Toxoplasma infection of humans was analyzed by immunoglobulin G (IgG), IgA, and IgM enzyme-linked immunosorbent assay with Rop2196-561 as the antigen substrate. The analyzed sera were divided according to T. gondii-specific serological tests (IgG, IgA, or IgM indirect immunofluorescence and IgA or IgM immunosorbent agglutination assay) as group A (IgG+ IgA- IgM-; n = 35), group B (IgG+ IgA+ IgM+; n = 21), group C (IgG+ IgA+ IgM-; n = 5), and group D (IgG+ IgA- IgM+; n = 16). Twenty-six T. gondii-seronegative sera from individuals with other infections were also included (group E). Anti-Rop2 IgG antibodies were detected in 82.8% of group A sera and in 97.6% of the sera with acute-phase marker immunoglobulins (groups B, C, and D). The percentage of IgA antibody reactivity against Rop2196-561 was 17.1% in group A, 50% in group D, and 80.8% in groups B and C. The percentage of IgM antibody reactivity was 0% in groups A and C and 62% in groups B and D. Sera from group E failed to show IgA, IgM, or IgG antibody reactivity. Since T. gondii Rop2 elicits a strong humoral response from an early stage of infection, it is suggested that recombinant Rop2196-561 would be suitable for use in diagnostic systems, in combination with other T. gondii antigens, to detect specific IgG, IgA, and IgM antibodies.

Fil: Martin, Valentina. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.

Fil: Arcavi, Miriam. Universidad de Buenos Aires. Immunología Clínica; Argentina.

Fil: Santillan, Graciela. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.

Fil: Amendoeira, María Regina R. Fundaçao Oswaldo Cruz. Instituto Oswaldo Cruz; Brazil.

Fil: De Souza Neves, Elizabeht. Hospital Evandro Chagas-FIOCRUZ; Brazil.

Fil: Griemberg, Gloria. Universidad de Buenos Aires. Immunología Clínica; Argentina.

Fil: Guarnera, Eduardo. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.

Fil: Garberi, Juan C. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.

Fil: Angel, Sergio O. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.
URI: http://sgc.anlis.gob.ar/handle/123456789/392
http://cdli.asm.org/content/5/5/627.full.pdf+html
ISSN: 1098-6588
Rights: info:eu-repo/semantics/openAccess
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