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Please use this identifier to cite or link to this item: http://sgc.anlis.gob.ar/handle/123456789/392
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dc.contributor.authorMartin, Valentina-
dc.contributor.authorArcavi, Miriam-
dc.contributor.authorSantillan, Graciela-
dc.contributor.authorAmendoeira, María Regina R.-
dc.contributor.authorDe Souza Neves, Elizabeth-
dc.contributor.authorGriemberg, Gloria-
dc.contributor.authorGuarnera, Eduardo-
dc.contributor.authorGarberi, Juan C.-
dc.contributor.authorAngel, Sergio O.-
dc.date.accessioned2012-11-23T03:59:16Z-
dc.date.available2012-11-23T03:59:16Z-
dc.date.issued1998-
dc.identifier.issn1098-6588-
dc.identifier.urihttp://sgc.anlis.gob.ar/handle/123456789/392-
dc.identifier.urihttp://cdli.asm.org/content/5/5/627.full.pdf+html-
dc.descriptionThe Toxoplasma gondii rhoptry protein Rop2 was expressed in Escherichia coli as a fusion protein containing 44 kDa of the 55-kDa mature Rop2, supplied with six histidyl residues at the N-terminal end (Rop2196-561). Humoral response during Toxoplasma infection of humans was analyzed by immunoglobulin G (IgG), IgA, and IgM enzyme-linked immunosorbent assay with Rop2196-561 as the antigen substrate. The analyzed sera were divided according to T. gondii-specific serological tests (IgG, IgA, or IgM indirect immunofluorescence and IgA or IgM immunosorbent agglutination assay) as group A (IgG+ IgA- IgM-; n = 35), group B (IgG+ IgA+ IgM+; n = 21), group C (IgG+ IgA+ IgM-; n = 5), and group D (IgG+ IgA- IgM+; n = 16). Twenty-six T. gondii-seronegative sera from individuals with other infections were also included (group E). Anti-Rop2 IgG antibodies were detected in 82.8% of group A sera and in 97.6% of the sera with acute-phase marker immunoglobulins (groups B, C, and D). The percentage of IgA antibody reactivity against Rop2196-561 was 17.1% in group A, 50% in group D, and 80.8% in groups B and C. The percentage of IgM antibody reactivity was 0% in groups A and C and 62% in groups B and D. Sera from group E failed to show IgA, IgM, or IgG antibody reactivity. Since T. gondii Rop2 elicits a strong humoral response from an early stage of infection, it is suggested that recombinant Rop2196-561 would be suitable for use in diagnostic systems, in combination with other T. gondii antigens, to detect specific IgG, IgA, and IgM antibodies.ES
dc.descriptionFil: Martin, Valentina. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.ES
dc.descriptionFil: Arcavi, Miriam. Universidad de Buenos Aires. Immunología Clínica; Argentina.ES
dc.descriptionFil: Santillan, Graciela. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.ES
dc.descriptionFil: Amendoeira, María Regina R. Fundaçao Oswaldo Cruz. Instituto Oswaldo Cruz; Brazil.ES
dc.descriptionFil: De Souza Neves, Elizabeht. Hospital Evandro Chagas-FIOCRUZ; Brazil.ES
dc.descriptionFil: Griemberg, Gloria. Universidad de Buenos Aires. Immunología Clínica; Argentina.ES
dc.descriptionFil: Guarnera, Eduardo. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.ES
dc.descriptionFil: Garberi, Juan C. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.ES
dc.descriptionFil: Angel, Sergio O. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.ES
dc.formatapplication/pdfES
dc.language.isoengen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.sourceClinical and Diagnostic Laboratory Immunology, 1998, 5(5), 627-631.en_US
dc.subjectToxoplasmosisen_US
dc.subjectHumanosen_US
dc.subjectInmunoglobulina Aen_US
dc.subjectInmunoglobulina Men_US
dc.subjectInmunoglobulina Gen_US
dc.titleDetection of human Toxoplasma-specific immunoglobulins A, M, and G with a recombinant Toxoplasma gondii rop2 proteinen_US
dc.typeArtículoes
anlis.essnrd1es
item.openairetypeArtículo-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
crisitem.author.deptAdministración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS)-
crisitem.author.deptInstituto Nacional de Enfermedades Infecciosas (INEI)-
crisitem.author.deptDepartamento de Parasitología-
crisitem.author.deptMaestría en Microbiología Molecular UNSM-ANLIS-
crisitem.author.deptAdministración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS)-
crisitem.author.deptInstituto Nacional de Parasitología (INP)-
crisitem.author.parentorgAdministración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS)-
crisitem.author.parentorgInstituto Nacional de Enfermedades Infecciosas (INEI)-
crisitem.author.parentorgCentro Nacional Red de Laboratorios (CNRL)-
crisitem.author.parentorgAdministración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS)-
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