Please use this identifier to cite or link to this item:
http://sgc.anlis.gob.ar/handle/123456789/2535
Title: | Carbapenemase-Producing Extraintestinal Pathogenic Escherichia coli From Argentina: Clonal Diversity and Predominance of Hyperepidemic Clones CC10 and CC131 | Authors: | Sanz, María Belén De Belder, Denise de Mendieta, J M Faccone, Diego Poklépovich, Tomás Javier Lucero, María Celeste Rapoport, Melina J. Campos, Josefina Tuduri, Ezequiel Saavedra, Mathew O van der Ploeg, Claudia Andrea Rogé, Ariel Diego Pasteran, Fernando Corso, Alejandra Rosato, Adriana E Gómez, Sonia Alejandra |
Keywords: | Escherichia coli;Enterobacteriaceae Resistentes a los Carbapenémicos;Células Clonales;Filogenia | Issue Date: | 2022 | Journal: | Frontiers in microbiology | Series/Report no.: | Front Microbiol;18(13)2022:1-11 | Abstract: | Extraintestinal pathogenic Escherichia coli (ExPEC) causes infections outside the intestine. Particular ExPEC clones, such as clonal complex (CC)/sequence type (ST)131, have been known to sequentially accumulate antimicrobial resistance that starts with chromosomal mutations against fluoroquinolones, followed with the acquisition of bla CTX-M-15 and, more recently, carbapenemases. Here we aimed to investigate the distribution of global epidemic clones of carbapenemase-producing ExPEC from Argentina in representative clinical isolates recovered between July 2008 and March 2017. Carbapenemase-producing ExPEC (n = 160) were referred to the Argentinean reference laboratory. Of these, 71 were selected for genome sequencing. Phenotypic and microbiological studies confirmed the presence of carbapenemases confirmed as KPC-2 (n = 52), NDM-1 (n = 16), IMP-8 (n = 2), and VIM-1 (n = 1) producers. The isolates had been recovered mainly from urine, blood, and abdominal fluids among others, and some were from screening samples. After analyzing the virulence gene content, 76% of the isolates were considered ExPEC, although non-ExPEC isolates were also obtained from extraintestinal sites. Pan-genome phylogeny and clonal analysis showed great clonal diversity, although the first phylogroup in abundance was phylogroup A, harboring CC10 isolates, followed by phylogroup B2 with CC/ST131, mostly H30Rx, the subclone co-producing CTX-M-15. Phylogroups D, B1, C, F, and E were also detected with fewer strains. CC10 and CC/ST131 were found throughout the country. In addition, CC10 nucleated most metalloenzymes, such as NDM-1. Other relevant international clones were identified, such as CC/ST38, CC155, CC14/ST1193, and CC23. Two isolates co-produced KPC-2 and OXA-163 or OXA-439, a point mutation variant of OXA-163, and three isolates co-produced MCR-1 among other resistance genes. To conclude, in this work, we described the molecular epidemiology of carbapenemase-producing ExPEC in Argentina. Further studies are necessary to determine the plasmid families disseminating carbapenemases in ExPEC in this region. |
Description: | Fil: Sanz, María Belén. Servicio Antimicrobianos, Laboratorio Nacional de Referencia en Resistencia a los Antimicrobianos (LNRRA), INEI-ANLIS "Dr. Carlos G. Malbrán"; Buenos Aires, Argentina Fil: De Belder, Denise. Plataforma Genómica y Bioinformática (PLABIO), INEI-ANLIS "Dr. Carlos G. Malbrán"; Buenos Aires, Argentina Fil: De Mendieta, J M. Servicio Antimicrobianos, Laboratorio Nacional de Referencia en Resistencia a los Antimicrobianos (LNRRA), INEI-ANLIS "Dr. Carlos G. Malbrán"; Buenos Aires, Argentina Fil: Faccone, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Buenos Aires, Argentina Fil: Poklepovich, Tomás. Plataforma Genómica y Bioinformática (PLABIO), INEI-ANLIS "Dr. Carlos G. Malbrán"; Buenos Aires, Argentina Fil: Lucero, Celeste. Servicio Antimicrobianos, Laboratorio Nacional de Referencia en Resistencia a los Antimicrobianos (LNRRA), INEI-ANLIS "Dr. Carlos G. Malbrán"; Buenos Aires, Argentina Fil: Rapoport, Melina. Servicio Antimicrobianos, Laboratorio Nacional de Referencia en Resistencia a los Antimicrobianos (LNRRA), INEI-ANLIS "Dr. Carlos G. Malbrán"; Buenos Aires, Argentina Fil: Campos, Josefina. Plataforma Genómica y Bioinformática (PLABIO), INEI-ANLIS "Dr. Carlos G. Malbrán"; Buenos Aires, Argentina Fil: Tuduri, Ezequiel. Servicio Antimicrobianos, Laboratorio Nacional de Referencia en Resistencia a los Antimicrobianos (LNRRA), INEI-ANLIS "Dr. Carlos G. Malbrán"; Buenos Aires, Argentina Fil: Saavedra, Mathew O. Department of Pathology and Genomic Medicine, Center for Molecular and Translational Human Infectious Diseases Research, Houston Methodist Hospital, Houston Methodist Research Institute; United States Fil: Van der Ploeg, Claudia. Servicio de Antígenos y Antisueros, INPB-ANLIS "Dr. Carlos G. Malbrán"; Buenos Aires, Argentina Fil: Rogé, Ariel. Servicio de Antígenos y Antisueros, INPB-ANLIS "Dr. Carlos G. Malbrán"; Buenos Aires, Argentina Fil: Pasteran, Fernando. Servicio Antimicrobianos, Laboratorio Nacional de Referencia en Resistencia a los Antimicrobianos (LNRRA), INEI-ANLIS "Dr. Carlos G. Malbrán"; Buenos Aires, Argentina Fil: Corso, Alejandra. Servicio Antimicrobianos, Laboratorio Nacional de Referencia en Resistencia a los Antimicrobianos (LNRRA), INEI-ANLIS "Dr. Carlos G. Malbrán"; Buenos Aires, Argentina Fil: Rosato, Adriana E. Department of Pathology and Molecular Microbiology Diagnostics-Research, Riverside University Health System; United States Fil: Gomez, Sonia A. Servicio Antimicrobianos, Laboratorio Nacional de Referencia en Resistencia a los Antimicrobianos (LNRRA), INEI-ANLIS "Dr. Carlos G. Malbrán"; Buenos Aires, Argentina |
URI: | http://sgc.anlis.gob.ar/handle/123456789/2535 | ISSN: | 1664-302X | DOI: | 10.3389/fmicb.2022.830209 |
Appears in Collections: | Artículos |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
830209.pdf | 3.77 MB | Adobe PDF | View/Open |
Page view(s)
56
checked on Oct 15, 2024
Download(s)
7
checked on Oct 15, 2024
Google ScholarTM
Check
Altmetric
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.