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Título: | Toxoplasma gondii protease inhibitor-1 (TgPI-1) is a novel vaccine candidate against toxoplasmosis | Autor(es): | Cuppari, Anahi Fernandez Sanchez, Vanesa Ledesma, Bibiana A Frank, Fernanda M. Goldman, Alejandra Angel, Sergio O. Martín, Valentina |
Palavras-chave: | Toxoplasma;Toxoplasmosis | Data do documento: | 15-Set-2008 | Editora: | Elsevier | Jornal: | Vaccine | Resumo: | The Toxoplasma gondii serin protease inhibitor-1 (TgPI-1) is a dense granule antigen that showed to specifically inhibit trypsin, chymotrypsin and neutrophil elastase, suggesting a possible modulatory role during the parasite invasion process and on the development of the innate immune response. To study the immune-protective value of TgPI-1, C3H/HeN mice were immunized with a recombinant form of the antigen rTgPI-1 combined with alum. All immunized mice produced specific anti-rTgPI-1 immunoglobulins, with high IgG antibody titers and a mixed IgG(1)/IgG(2a) response, with predominance of IgG(1) production. The cellular immune response was associated with the production of IFN-gamma and IL-10 cytokines. Vaccinated mice displayed significant protection against an oral challenge either after a lethal infection with Me49 cysts (90% survival vs. 50%) and also after a non-lethal infection (58% reduction in brain parasite load) compared to the non-vaccinated control group. In conclusion, rTgPI-1 elicits a strong specific immune response providing partial protection against both T. gondii acute and chronic infection, so it would be a good candidate in a vaccine against toxoplasmosis, which could be combined with other relevant parasite antigens. |
Descrição: | Fil: Cuppari, Anahi Fernandez. Laboratorio de Inmunología, Centro de Salud y Medio Ambiente, E.C.y T., Universidad de Gral. San Martín, Provincia de Buenos Aires; Argentina. Fil: Sanchez, Vanesa. Laboratorio de Inmunología, Centro de Salud y Medio Ambiente, E.C.y T., Universidad de Gral. San Martín, Provincia de Buenos Aires; Argentina. Fil: Ledesma, Bibiana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina. Fil: Frank, Fernanda M. Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, and Facultad de Farmacia y Bioquímica, IDEHU, CONICET Universidad de Buenos Aires, Buenos Aires; Argentina Fil: Goldman, Alejandra. Laboratorio de Inmunología, Centro de Salud y Medio Ambiente, E.C.y T., Universidad de Gral. San Martín, Provincia de Buenos Aires, Argentina. Fil: Angel, Sergio O. Laboratorio de Parasitología Molecular, IIB-INTECH, CONICET-UNSAM, Chascomús, Provincia de Buenos Aires, Argentina. Fil: Martin, Valentina. Laboratorio de Inmunología, Centro de Salud y Medio Ambiente, E.C.y T., Universidad de Gral. San Martín, Provincia de Buenos Aires; Argentina. |
URI: | http://sgc.anlis.gob.ar/handle/123456789/2253 | ISSN: | 0264-410X | DOI: | 10.1016/j.vaccine.2008.07.031 | Direitos: | Closed Access |
Aparece nas Coleções: | Publicaciones INEI |
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