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Title: | AA479 antiserum: new reagent for the serotype characterization of atypical variants of Shigella flexneri | Authors: | van der Ploeg, Claudia Andrea Rogé, Ariel Bordagorria, Ximena L. de Urquiza, María T. Viñas, María R. Pichel, Mariana Bruno, Susana B |
Keywords: | Serotipificación;Shigella flexneri;Variantes atípicas;Conejos | Issue Date: | 2015 | Project: | datasets | Journal: | Revista Argentina de microbiologia | Abstract: | Shigella flexneri is divided into 13 serotypes based on the combination of antigenic determinants present in the O-antigen. A new O-antigen modification with phosphoethanolamine has been identified. The presence of this antigenic determinant (called E1037) is recognized by monoclonal antibody MASF IV-1. Given the increasing incidence of these new variants and the difficulty in supplying the monoclonal antibody to our country, we produced a polyclonal antiserum (AA479) through immunization with a S. flexneri Xv strain. The antiserum specificity was assessed by slide agglutination against isolates from clinical cases and a culture collection representing all Shigella serotypes. The results obtained demonstrated a 100% correlation between AA479 absorbed antiserum and monoclonal antibody MASF IV-1. The availability of AA479 antiserum in every public hospital in Argentina will allow us to identify atypical S. flexneri isolates in order to strengthen Shigella surveillance in our country and to compare with global epidemiological data. |
Description: | Fil: van der Ploeg, Claudia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos. Servicio Antígenos y Antisueros; Argentina. Fil: Rogé, Ariel. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos. Servicio Antígenos y Antisueros; Argentina. Fil: Bordagorria, Ximena L. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos. Servicio Antígenos y Antisueros; Argentina. Fil: de Urquiza, María T. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos. Servicio Antígenos y Antisueros; Argentina. Fil: Viñas, María R. ANLIS Dr.C.G. Malbrán. Instituto Nacional de Enfermedades Infeccionsas. Servicio Enterobacterias; Argentina. Fil: Pichel, Mariana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infeccionsas. Servicio Enterobacterias; Argentina. Fil: Bruno, Susana B. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos. Servicio Antígenos y Antisueros; Argentina. |
URI: | http://sgc.anlis.gob.ar/handle/123456789/1786 | ISSN: | 0325-7541 | DOI: | 10.1016/j.ram.2014.11.001 | Rights: | Open Access Creative Commons Attribution 4.0 International License |
Appears in Collections: | Publicaciones INPB |
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RAM_2015_47_1_36-40.pdf | Artículo en inglés | 339.33 kB | Adobe PDF | View/Open |
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