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Title: | Direct molecular identification of Trypanosoma cruzi discrete typing units in domestic and peridomestic Triatoma infestans and Triatoma sordida from the Argentine Chaco | Authors: | Maffey, L Cardinal, Marta Victoria Ordóñez-Krasnowski, P C Lanati, L A Lauricella, Marta A. Schijman, A G Gürtler, Ricardo E. |
Keywords: | Animales;Animales Domésticos;Argentina;Perros;Ensayo de Inmunoadsorción Enzimática;Genotipo;Humanos;Insectos Vectores;Reacción en Cadena de la Polimerasa;Triatoma;Enfermedad de Chagas | Issue Date: | Oct-2012 | Publisher: | Cambridge University Press | Project: | datasets | Journal: | Parasitology | Abstract: | We assessed the distribution of Trypanosoma cruzi Discrete Typing Units (DTUs) in domestic and peridomestic Triatoma infestans and Triatoma sordida specimens collected in a well-defined rural area in Pampa del Indio, northeastern Argentina. Microscopically-positive bugs were randomly selected with a multi-level sampling design, and DTUs were identified using direct PCR strategies. TcVI predominated in 61% of 69 T. infestans and in 56% of 9 T. sordida. TcV was the secondary DTU in T. infestans (16%) and was found in 1 T. sordida specimen (11%). Three T. sordida (33%) were found infected with TcI, a DTU also identified in local Didelphis albiventris opossums. Mixed DTU infections occurred rarely (5%) and were detected both directly from the bugs' rectal ampoule and parasite cultures. The identified DTUs and bug collection sites of T. infestans were significantly associated. Bugs infected with TcV were almost exclusively captured in domiciles whereas those with TcVI were found similarly in domiciles and peridomiciles. All mixed infections occurred in domiciles. TcV-infected bugs fed more often on humans than on dogs, whereas TcVI-infected bugs showed the reverse pattern. T. sordida is a probable sylvatic vector of TcI linked to D. albiventris, and could represent a secondary vector of TcVI and TcV in the domestic/peridomestic cycle. |
Description: | Fil: Maffey, L. Universidad de Buenos Aires. Departamento de Ecología. Laboratorio de Eco-Epidemiología, Genética y Evolución; Argentina. Fil: Cardinal, M. B. Universidad de Buenos Aires. Departamento de Ecología. Laboratorio de Eco-Epidemiología, Genética y Evolución; Argentina. Fil: Ordóñez-Krasnowski, P. C. Universidad de Buenos Aires. Departamento de Ecología. Laboratorio de Eco-Epidemiología, Genética y Evolución; Argentina. Fil: Lanati, L. A. Universidad de Buenos Aires. Departamento de Ecología. Laboratorio de Eco-Epidemiología, Genética y Evolución; Argentina. Fil: Lauricella, M. A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina. Fil: Schijman, A. G. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular. Laboratorio de Biología Molecular de la Enfermedad de Chagas; Argentina. Fil: Gürtler, R. E. Universidad de Buenos Aires. Departamento de Ecología. Laboratorio de Eco-Epidemiología, Genética y Evolución; Argentina. |
URI: | http://sgc.anlis.gob.ar/handle/123456789/1684 | DOI: | 10.1017/S0031182012000856 | Rights: | Open Access |
Appears in Collections: | Publicaciones INP |
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nihms493184.pdf | Artículo en inglés | 584.96 kB | Adobe PDF | View/Open |
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