Por favor, use este identificador para citar o enlazar este ítem:
http://sgc.anlis.gob.ar/handle/123456789/1654
Título : | Characterization of a basic phospholipase A2-homologeu myotoxin isolated from the venom of the snake Bothrops neuwiedii (yarará chica) from Argentina | Autor : | Geoghegan, Patricia A. Angulo, Yamileth Cangelosi, Adriana Díaz, Mónica Lomonte, Bruno |
Palabras clave : | Venenos de Serpiente;Argentina;Fosfolipasas A2;Edema;Bothrops | Fecha de publicación : | dic-1999 | Journal: | Toxicon : official journal of the International Society on Toxinology | Resumen : | A basic protein was isolated by CM-Sephadex C-25 chromatography from the venom of Bothrops neuwiedii from Argentina, and named B. neuwiedii myotoxin I. This protein exerted local myotoxic and edema-forming effects in mice, with potencies comparable to other myotoxins isolated from Bothrops spp. venoms. When injected by i.v. route at doses up to 4.7 mg/kg of body weight, the toxin was not lethal. In vitro, the toxin had no detectable phospholipase A2 activity on egg yolk phospholipids. B. neuwiedii myotoxin I appeared as a homodimer in sodium dodecylsulphate-polyacrylamide gel electrophoresis, with a subunit molecular weight of 15 kD. Gel immunodiffusion revealed a pattern of partial antigenic identity between the newly isolated myotoxin and myotoxin II from Bothrops asper venom. The sequence of B. neuwiedii myotoxin I was determined for the first 40 amino acid residues, showing high homology to several class II phospholipase A2 myotoxins of the Lys-49 family from crotalids. Altogether, results suggest that this toxin is a new member of the Lys-49 phospholipase A2-homologues with myotoxic, cytolytic, and edema-inducing activities. |
Descripción : | Fil: Geoghegan, Patricia A. ANLIS Dr.C.G.Malbrán. Centro Nacional de Control de Calidad de Biológicos. Servicio de Inmunoterapéuticos; Argentina. Fil: Angulo, Yamileth. Universidad de Costa Rica. Facultad de Microbiología. Instituto Clodomiro Picado; Costa Rica. Fil: Cangelosi, Adriana. ANLIS Dr.C.G.Malbrán. Centro Nacional de Control de Calidad de Biológicos. Servicio de Inmunoterapéuticos; Argentina. Fil: Díaz, Mónica. ANLIS Dr.C.G.Malbrán. Centro Nacional de Control de Calidad de Biológicos. Servicio de Inmunoterapéuticos; Argentina. Fil: Lomonte, Bruno. Universidad de Costa Rica. Facultad de Microbiología. Instituto Clodomiro Picado; Costa Rica. |
URI : | http://sgc.anlis.gob.ar/handle/123456789/1654 | ISSN : | 0041-0101 | DOI: | 10.1016/s0041-0101(99)00115-4 | Derechos: | Closed Access |
Aparece en las colecciones: | Publicaciones CNCCB |
Mostrar el registro Dublin Core completo del ítem
Visualizaciones de página(s)
63
comprobado en 09-ago-2024
Google ScholarTM
Consultar
Altmetric
Altmetric
Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.