Please use this identifier to cite or link to this item:
http://sgc.anlis.gob.ar/handle/123456789/1654
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Geoghegan, Patricia A. | es |
dc.contributor.author | Angulo, Yamileth | es |
dc.contributor.author | Cangelosi, Adriana | es |
dc.contributor.author | Díaz, Mónica | es |
dc.contributor.author | Lomonte, Bruno | es |
dc.date.accessioned | 2020-09-17T01:20:54Z | - |
dc.date.available | 2020-09-17T01:20:54Z | - |
dc.date.issued | 1999-12 | - |
dc.identifier.issn | 0041-0101 | - |
dc.identifier.uri | http://sgc.anlis.gob.ar/handle/123456789/1654 | - |
dc.description | Fil: Geoghegan, Patricia A. ANLIS Dr.C.G.Malbrán. Centro Nacional de Control de Calidad de Biológicos. Servicio de Inmunoterapéuticos; Argentina. | es |
dc.description | Fil: Angulo, Yamileth. Universidad de Costa Rica. Facultad de Microbiología. Instituto Clodomiro Picado; Costa Rica. | es |
dc.description | Fil: Cangelosi, Adriana. ANLIS Dr.C.G.Malbrán. Centro Nacional de Control de Calidad de Biológicos. Servicio de Inmunoterapéuticos; Argentina. | es |
dc.description | Fil: Díaz, Mónica. ANLIS Dr.C.G.Malbrán. Centro Nacional de Control de Calidad de Biológicos. Servicio de Inmunoterapéuticos; Argentina. | es |
dc.description | Fil: Lomonte, Bruno. Universidad de Costa Rica. Facultad de Microbiología. Instituto Clodomiro Picado; Costa Rica. | es |
dc.description.abstract | A basic protein was isolated by CM-Sephadex C-25 chromatography from the venom of Bothrops neuwiedii from Argentina, and named B. neuwiedii myotoxin I. This protein exerted local myotoxic and edema-forming effects in mice, with potencies comparable to other myotoxins isolated from Bothrops spp. venoms. When injected by i.v. route at doses up to 4.7 mg/kg of body weight, the toxin was not lethal. In vitro, the toxin had no detectable phospholipase A2 activity on egg yolk phospholipids. B. neuwiedii myotoxin I appeared as a homodimer in sodium dodecylsulphate-polyacrylamide gel electrophoresis, with a subunit molecular weight of 15 kD. Gel immunodiffusion revealed a pattern of partial antigenic identity between the newly isolated myotoxin and myotoxin II from Bothrops asper venom. The sequence of B. neuwiedii myotoxin I was determined for the first 40 amino acid residues, showing high homology to several class II phospholipase A2 myotoxins of the Lys-49 family from crotalids. Altogether, results suggest that this toxin is a new member of the Lys-49 phospholipase A2-homologues with myotoxic, cytolytic, and edema-inducing activities. | es |
dc.language.iso | en | es |
dc.relation.ispartof | Toxicon : official journal of the International Society on Toxinology | es |
dc.rights | Closed Access | - |
dc.subject | Venenos de Serpiente | es |
dc.subject | Argentina | es |
dc.subject | Fosfolipasas A2 | es |
dc.subject | Edema | es |
dc.subject | Bothrops | es |
dc.title | Characterization of a basic phospholipase A2-homologeu myotoxin isolated from the venom of the snake Bothrops neuwiedii (yarará chica) from Argentina | es |
dc.type | Artículo | es |
dc.identifier.doi | 10.1016/s0041-0101(99)00115-4 | - |
anlis.essnrd | 1 | - |
item.cerifentitytype | Publications | - |
item.openairetype | Artículo | - |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | No Fulltext | - |
item.grantfulltext | none | - |
crisitem.author.dept | Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS) | - |
crisitem.author.dept | Centro Nacional de Control de Calidad de Biológicos (CNCCB) | - |
crisitem.author.dept | Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS) | - |
crisitem.author.dept | Centro Nacional de Control de Calidad de Biológicos (CNCCB) | - |
crisitem.author.parentorg | Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS) | - |
crisitem.author.parentorg | Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS) | - |
Appears in Collections: | Publicaciones CNCCB |
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