Please use this identifier to cite or link to this item: http://sgc.anlis.gob.ar/handle/123456789/1501
Title: Molecular cloning, sequencing and expression of a serine proteinase inhibitor gene from Toxoplasma gondii
Authors: Pszenny, Viviana 
Angel, Sergio O. 
Duschak, Vilma G 
Paulino, M 
Ledesma, Bibiana A 
Yabo, M I 
Guarnera, Eduardo 
Ruiz, Andrés M 
Bontempi, Esteban 
Keywords: Inhibidor de Serinpeptidasas Tipo Kazal-5;Inhibidores de Serinpeptidasas Tipo Kazal;Toxoplasma
Issue Date: 15-Apr-2000
Journal: Molecular and biochemical parasitology 
Abstract: 
A cDNA clone from a Toxoplasma gondii tachyzoite cDNA library encoding a serine proteinase inhibitor (serpin) was isolated. The 1376 bp cDNA sequence encodes a 294 amino acid protein with a putative signal peptide of 23 amino acids resulting in a mature protein with a predicted mass of 30,190 Da and a pI of 4.86. This protein has internal sequence similarity of residues 30-66, 114-150, 181-217 and 247-283 indicating a four-domain structure. The four domains exhibit high identity to serine proteinase inhibitors belonging to the non-classical Kazal-type family. The gene is single copy in the tachyzoite haploid genome of RH strain and was amplified by polymerase chain reaction (PCR). Several introns were identified. The sequence encoding the mature protein was amplified by PCR, cloned into the pQE30 vector and expressed in Escherichia coli. Specific antiserum generated against the recombinant protein was used in immunoblot assay and two bands of 38 and 42 kDa were detected in a whole parasite homogenate. The recombinant protein showed trypsin-inhibitory activity, one of the two potential specificities. We discuss the possible roles that T. gondii serpin(s) may play in the survival of the tachyzoites in the host.
Description: 
Fil: Pszenny, Viviana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.

Fil: Angel, Sergio O. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.

Fil: Duschak, Vilma G. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina.

Fil: Paulino, Margot. Universidad de la República. Facultad de Química. Cátedra de Química Cuántica, Montevideo; Uruguay.

Fil: Ledesma, Bibiana A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.

Fil: Yabo, Miriam I. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.

Fil: Guarnera, Eduardo. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.

Fil: Ruiz, Andrés M. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.

Fil: Bontempi, Esteban. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.
URI: http://sgc.anlis.gob.ar/handle/123456789/1501
ISSN: 0166-6851
DOI: 10.1016/s0166-6851(00)00202-4
Rights: Closed Access
Appears in Collections:Publicaciones INP

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