Please use this identifier to cite or link to this item:
Title: Distinct Treatment Outcomes of Antiparasitic Therapy in Trypanosoma cruzi-Infected Children Is Associated With Early Changes in Cytokines, Chemokines, and T-Cell Phenotypes
Authors: Albareda, María Cecilia 
Natale, María A 
De Rissio, Ana María 
Fernandez, Marisa 
Serjan, Alicia 
Alvarez, María G 
Cooley, Gretchen 
Shen, Huifeng 
Viotti, Rodolfo 
Bua, Jacqueline 
Castro Eiro, Melisa D 
Nuñez, Myriam 
Fichera, Laura E. 
Lococo, Bruno 
Scollo, Karenina 
Tarleton, Rick L 
Laucella, Susana A. 
Keywords: Linfocitos T;Trypanosoma cruzi;benznidazole;Nifurtimox;infección pediátrica
Issue Date: 2018
Journal: Frontiers in immunology 
Background: In contrast to adults, Trypanosoma cruzi-infected children have more broadly functional Trypanosoma cruzi-specific T cells, and the total T-cell compartment exhibits fewer signs of immune exhaustion. However, not much is known about the link between immunocompetence and the treatment efficacy for human Chagas disease. Methods: Using cytokine enzyme-linked immunosorbent spot (ELISPOT) polychromatic flow cytometry, cytometric bead assay, multiplex serological assays and quantitative PCR, we evaluated T. cruzi-specific T-cell and antibody immune responses, T-cell phenotypes and parasitemia in children in the early chronic phase of Chagas disease undergoing anti-Trypanosoma cruzi treatment. Results: Treatment with benznidazole or nifurtimox induced a decline in T. cruzi-specific IFN-γ- and IL-2-producing cells and proinflammatory cytokines and chemokines. T-cell responses became detectable after therapy in children bearing T-cell responses under background levels prior to treatment. The total frequencies of effector, activated and antigen-experienced T cells also decreased following anti-T. cruzi therapy, along with an increase in T cells expressing the receptor of the homeostatic cytokine IL-7. Posttreatment changes in several of these markers distinguished children with a declining serologic response suggestive of successful treatment from those with sustained serological responses in a 5-year follow-up study. A multivariate analysis demonstrated that lower frequency of CD4+CD45RA-CCR7-CD62L- T cells prior to drug therapy was an independent indicator of successful treatment. Conclusions: These findings further validate the usefulness of alternative metrics to monitor treatment outcomes. Distinct qualitative and quantitative characteristics of T cells prior to drug therapy may be linked to treatment efficacy.
DOI: 10.3389/fimmu.2018.01958
Appears in Collections:Publicaciones INP

Files in This Item:
File Description SizeFormat
fimmu-09-01958.pdfArtículo en inglés3.82 MBAdobe PDFThumbnail
Show full item record

Page view(s)

checked on Jun 26, 2022


checked on Jun 26, 2022

Google ScholarTM




Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.