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Title: | Redirection of the Immune Response to the Functional Catalytic Domain of the Cystein Proteinase Cruzipain Improves Protective Immunity against Trypanosoma cruzi Infection | Authors: | Cazorla, Silvia I. Frank, Fernanda M. Becker, Pablo D. Arnaiz, María Rosa Mirkin, Gerardo A. Corral, Ricardo S. Guzman, Carlos A. Malchiodi, Emilio L. |
Keywords: | Trypanosoma cruzi;Enfermedad de Chagas;Endopeptidasas;Péptido Hidrolasas | Issue Date: | 1-Jul-2010 | Publisher: | Oxford University Press | Journal: | The Journal of infectious diseases | Abstract: | Despite the strong immune responses elicited after natural infection with Trypanosoma cruzi or vaccination against it, parasite survival suggests that these responses are insufficient or inherently inadequate. T. cruzi contains a major cystein proteinase, cruzipain, which has a catalytic N-terminal domain and a C-terminal extension. Immunizations that employed recombinant cruzipain or its N- and C-terminal domains allowed evaluation of the ability of cruzipain to circumvent responses against the catalytic domain. This phenomenon is not a property of the parasite but of cruzipain itself, because recombinant cruzipain triggers a response similar to that of cruzipain during natural or experimental infection. Cruzipain is not the only antigen with a highly immunogenic region of unknown function that somehow protects an essential domain for parasite survival. However, our studies show that this can be reverted by using the N- terminal domain as a tailored immunogen able to redirect host responses to provide enhanced protection. |
Description: | Fil: Cazorla, Silvia I. Cátedra de Inmunología and Instituto de Estudios de la Inmunidad Humoral (IDEHU), CONICET-UBA, Facultad de Farmacia y Bioquímica, Buenos Aires; Argentina. Fil: Frank, Fernanda M. Cátedra de Inmunología and Instituto de Estudios de la Inmunidad Humoral (IDEHU), CONICET-UBA, Facultad de Farmacia y Bioquímica, Buenos Aires; Argentina. Fil: Becker, Pablo D. Department of Vaccinology and Applied Microbiology, Helmholtz Centre for Infection Research, InhoffenstraΒe 7, D-38124 Braunschweig; Alemania. Fil: Arnaiz, María Rosa. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina. Fil: Mirkin, Gerardo A. Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires; Argentina. Fil: Corral, Ricardo S. Servicio de Parasitología-Chagas, Hospital de Niños Ricardo Gutiérrez, Buenos Aires; Argentina. Fil: Guzman, Carlos A. Department of Vaccinology and Applied Microbiology, Helmholtz Centre for Infection Research, InhoffenstraΒe 7, D-38124 Braunschweig; Alemania. Fil: Malchiodi, Emilio L. Cátedra de Inmunología and Instituto de Estudios de la Inmunidad Humoral (IDEHU), CONICET-UBA, Facultad de Farmacia y Bioquímica, Buenos Aires; Argentina. |
URI: | http://sgc.anlis.gob.ar/handle/123456789/533 | ISSN: | 1537-6613 | DOI: | 10.1086/652872. | Rights: | info:eu-repo/semantics/openAccess |
Appears in Collections: | Preproducción Publicaciones INP |
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File | Description | Size | Format | |
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JournalofInfectiousDiseases,2010,202(1),136-144..pdf | Artículo en inglés | 5.09 MB | Adobe PDF | View/Open |
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