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Título: | CS22, a novel human enterotoxigenic Escherichia coli adhesin, is related to CS15 | Autor(es): | Pichel, Mariana Binsztein, Norma Viboud, Gloria I. |
Palavras-chave: | Escherichia coli;Escherichia coli Enterotoxigénica;Proteínas de Escherichia coli | Data do documento: | 2000 | Descrição: | Enterotoxigenic Escherichia coli (ETEC) expresses a broad spectrum of O:H antigens. Serogroup O20 is one of the most prevalent among the ETEC strains lacking any of the defined colonization factors (CFs), in Argentina. An O20:H− strain, ARG-3, adhered to Caco-2 cells and exhibited a thermoregulated 15.7-kDa protein band upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). An antiserum against this protein inhibited ARG-3 adhesion to Caco-2 cells and bound to very thin fibrilla-like structures on the bacterial surface. A 15.7-kDa protein-defective mutant failed to adhere to Caco-2 cells and lacked immunogold-labeled surface structures. The N-terminal amino acid sequence of the structural subunit showed 95% homology to that of CS15 of ETEC (former antigen 8786) and 65% homology with fimbria SEF14 of Salmonella enterica serovar Enteritidis. Nevertheless, the molecular size of ARG-3 adhesin was different from that of CS15, as revealed by SDS-PAGE and mass spectrometry. Both proteins are immunologically related, yet not identical, since an antiserum against the 15.7-kDa protein reacted solely with ARG-3 after absorption with bacteria bearing CS15. Moreover, only under low stringency conditions could DNA from strain ARG-3 be amplified by PCR using primers derived from the nfaA sequence of CS15. Thus, from the DNA sequence obtained from the ARG-3 PCR product, it could be deduced that the subunit protein differed in 30 residues from that of CS15. ARG-3 adhesin was found in 60% of the O20:H- CF-negative ETEC strains from Argentina; however, it appeared restricted to this serotype. We propose the designation CS22 for the herein identified nonfimbrial adhesin of human ETEC. Fil: Pichel, Mariana G. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología; Argentina. Fil: Binsztein, Norma. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología; Argentina. Fil: Viboud, Gloria I. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología; Argentina. |
URI: | http://sgc.anlis.gob.ar/handle/123456789/409 http://iai.asm.org/content/68/6/3280.full.pdf |
ISSN: | 0019-9567 | Direitos: | 2000-12-31 info:eu-repo/semantics/embargoedAccess |
Aparece nas Coleções: | snrd Publicaciones INEI |
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Arquivo | Descrição | Tamanho | Formato | |
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InfectionandImmunity,2000,68(6),3280-3285.pdf | 2.68 MB | Adobe PDF | Ver/Aberto |
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