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dc.contributor.authorPichel, Mariana-
dc.contributor.authorBinsztein, Norma-
dc.contributor.authorViboud, Gloria I.-
dc.date.accessioned2012-11-25T22:02:39Z-
dc.date.available2012-11-25T22:02:39Z-
dc.date.issued2000-
dc.identifier.issn0019-9567-
dc.identifier.urihttp://sgc.anlis.gob.ar/handle/123456789/409-
dc.identifier.urihttp://iai.asm.org/content/68/6/3280.full.pdf-
dc.descriptionEnterotoxigenic Escherichia coli (ETEC) expresses a broad spectrum of O:H antigens. Serogroup O20 is one of the most prevalent among the ETEC strains lacking any of the defined colonization factors (CFs), in Argentina. An O20:H− strain, ARG-3, adhered to Caco-2 cells and exhibited a thermoregulated 15.7-kDa protein band upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). An antiserum against this protein inhibited ARG-3 adhesion to Caco-2 cells and bound to very thin fibrilla-like structures on the bacterial surface. A 15.7-kDa protein-defective mutant failed to adhere to Caco-2 cells and lacked immunogold-labeled surface structures. The N-terminal amino acid sequence of the structural subunit showed 95% homology to that of CS15 of ETEC (former antigen 8786) and 65% homology with fimbria SEF14 of Salmonella enterica serovar Enteritidis. Nevertheless, the molecular size of ARG-3 adhesin was different from that of CS15, as revealed by SDS-PAGE and mass spectrometry. Both proteins are immunologically related, yet not identical, since an antiserum against the 15.7-kDa protein reacted solely with ARG-3 after absorption with bacteria bearing CS15. Moreover, only under low stringency conditions could DNA from strain ARG-3 be amplified by PCR using primers derived from the nfaA sequence of CS15. Thus, from the DNA sequence obtained from the ARG-3 PCR product, it could be deduced that the subunit protein differed in 30 residues from that of CS15. ARG-3 adhesin was found in 60% of the O20:H- CF-negative ETEC strains from Argentina; however, it appeared restricted to this serotype. We propose the designation CS22 for the herein identified nonfimbrial adhesin of human ETEC.ES
dc.descriptionFil: Pichel, Mariana G. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología; Argentina.ES
dc.descriptionFil: Binsztein, Norma. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología; Argentina.ES
dc.descriptionFil: Viboud, Gloria I. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología; Argentina.ES
dc.formatapplication/pdfES
dc.language.isoengen_US
dc.rights2000-12-31en_US
dc.rightsinfo:eu-repo/semantics/embargoedAccessen_US
dc.sourceInfection and Immunity, 2000, 68(6), 3280-3285.en_US
dc.subjectEscherichia colien_US
dc.subjectEscherichia coli Enterotoxigénicaen_US
dc.subjectProteínas de Escherichia colien_US
dc.titleCS22, a novel human enterotoxigenic Escherichia coli adhesin, is related to CS15en_US
dc.typeArtículoes
anlis.essnrd1es
item.fulltextWith Fulltext-
item.openairetypeArtículo-
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.languageiso639-1en-
crisitem.author.deptAdministración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS)-
crisitem.author.deptInstituto Nacional de Enfermedades Infecciosas (INEI)-
crisitem.author.deptDepartamento de Bacteriología-
crisitem.author.deptServicio Enterobacterias-
crisitem.author.parentorgAdministración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS)-
crisitem.author.parentorgInstituto Nacional de Enfermedades Infecciosas (INEI)-
crisitem.author.parentorgDepartamento de Bacteriología-
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