Please use this identifier to cite or link to this item: http://sgc.anlis.gob.ar/handle/123456789/383
Title: Viable but nonculturable Vibrio cholerae O1 in the aquatic environment of Argentina
Authors: Binsztein, Norma 
Costagliola, Marcela C. 
Pichel, Mariana 
Jurquiza, Verónica 
Ramírez, Fernando C. 
Akselman, Rut 
Vacchino, Marta 
Huq, Anwarul 
Colwell, Rita 
Keywords: Vibrio cholerae O1;Toxinas Shiga;Agua de Mar;Cólera;Argentina
Issue Date: 2004
Publisher: American Society for Microbiology
Journal: Applied and Environmental Microbiology 
Abstract: 
In Argentina, as in other countries of Latin America, cholera has occurred in an epidemic pattern. Vibrio cholerae O1 is native to the aquatic environment, and it occurs in both culturable and viable but nonculturable (VNC) forms, the latter during interepidemic periods. This is the first report of the presence of VNC V. cholerae O1 in the estuarine and marine waters of the Rı´o de la Plata and the Argentine shelf of the Atlantic Ocean, respectively. Employing immunofluorescence and PCR methods, we were able to detect reservoirs of V. cholerae O1 carrying the virulence-associated genes ctxA and tcpA. The VNC forms of V. cholerae O1 were identified in samples of water, phytoplankton, and zooplankton; the latter organisms were mainly the copepods Acartia tonsa, Diaptomus sp., Paracalanus crassirostris, and Paracalanus parvus. We found that under favorable conditions, the VNC form of V. cholerae can revert to the pathogenic, transmissible state. We concluded that V. cholerae O1 is a resident of Argentinean waters, as has been shown to be the case in other geographic regions of the world.
Description: 
Fil: Binsztein, Norma. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología; Argentina.

Fil: Costagliola, Marcela C. Instituto Nacional de Investigación y Desarrollo Pesquero (INIDEP); Argentina.

Fil: Pichel, Mariana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología; Argentina.

Fil: Ramírez, Fernando C. Instituto Nacional de Investigación y Desarrollo Pesquero (INIDEP); Argentina.

Fil: Akselman, Rut. Instituto Nacional de Investigación y Desarrollo Pesquero (INIDEP); Argentina.

Fil: Vacchino, Marta. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Epidemiología; Argentina.

Fil: Huq, Anwarul. University of Maryland. Center of Marine Biotechnology; Estados Unidos.

Fil: Colwell, Rita. University of Maryland. Center of Marine Biotechnology; Estados Unidos.

Fil: Jurquiza, Verónica. Instituto Nacional de Investigacio´n y Desarrollo Pesquero (INIDEP); Argentina.
URI: http://sgc.anlis.gob.ar/handle/123456789/383
https://aem.asm.org/content/aem/70/12/7481.full.pdf
ISSN: 1098-5336
Rights: Closed Access
Appears in Collections:snrd
Publicaciones INEI
Publicaciones INE

Show full item record

Page view(s)

22
checked on Oct 25, 2021

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.