Please use this identifier to cite or link to this item: http://sgc.anlis.gob.ar/handle/123456789/369
Title: Multiple antibiotic-resistance mechanisms including a novel combination of extended-spectrum beta-lactamases in a Klebsiella pneumoniae clinical strain isolated in Argentina
Authors: Melano, Roberto 
Corso, Alejandra 
Petroni, Alejandro 
Centrón, Daniela 
Orman, Betina 
Pereyra, Adriana 
Moreno, Noemí 
Galas, Marcelo F. 
Keywords: Farmacorresistencia Bacteriana Múltiple;Klebsiella pneumoniae;beta-Lactamasas;Argentina
Issue Date: 2003
Description: Klebsiella pneumoniae M1803, isolated from a paediatric patient with chronic urinary infection, presented nine antimicrobial resistance mechanisms harboured on two conjugative megaplasmids, in addition to the chromosomally mediated SHV-1 beta-lactamase. These nine antimicrobial resistance mechanisms comprised two extended-spectrum beta-lactamases (ESBLs) (PER-2 and CTX-M-2), TEM-1-like, OXA-9-like, AAC(3)-IIa, AAC(6')-Ib, ANT(3")-Ia and resistance determinants to tetracycline and chloramphenicol. During fluoroquinolone treatment, a variant derived from M1803 (named M1826) was selected, with an overall increase of MICs, in particular of cefoxitin and carbapenems. No enzymic activity against these latter drugs was found. Mutations in the region analogous to the quinolone resistance-determining region were not found. Strain M1826 was deficient in OmpK35/36 expression, which produced the decrease in the susceptibility to cefoxitin, carbapenems and fluoroquinolones. The blaCTX-M-2 gene was located in an unusual class 1 integron, which includes Orf513, as occurred in the recently described In35. In addition, Tn3 and Tn1331 were detected in both K. pneumoniae isolates. This is the first report of in vivo selection of an OmpK35/36 deficiency in a K. pneumoniae strain that produced a novel combination of two ESBLs (CTX-M-2 and PER-2) during fluoroquinolone treatment in a paediatric patient with chronic urinary infection.
Fil: Melano, Roberto. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio de Antimicrobianos; Argentina.
Fil: Corso, Alejandra. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio de Antimicrobianos; Argentina.
Fil: Petroni, Alejandro. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio de Antimicrobianos; Argentina.
Fil: Centrón, Daniela. Universidad de Buenos Aires. Departamento de Microbiología; Argentina.
Fil: Orman, Betina. Universidad de Buenos Aires. Departamento de Microbiología; Argentina.
Fil: Pereyra, Adriana. Hospital Gobernador Centeno. Servicio de Bacteriología; Argentina.
Fil: Moreno, Noemí. Hospital Gobernador Centeno. Servicio de Bacteriología; Argentina.
Fil: Galas, Marcelo F. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio de Antimicrobianos; Argentina.
URI: http://jac.oxfordjournals.org/content/52/1/36.full.pdf+html
http://sgc.anlis.gob.ar/handle/123456789/369
ISSN: 1460-2091
Rights: 2004-05-29
info:eu-repo/semantics/embargoedAccess
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