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Title: | Peptidome profiling for the immunological stratification in sepsis: a proof of concept study | Authors: | Ledesma, Martín Todero, María Florencia Maceira, Lautaro Prieto, Mónica A. Vay, Carlos A Galas, Marcelo F. López, Beatriz Yokobori, Noemi Rearte, Bárbara |
Keywords: | Sepsis;Lipopolisacáridos | Issue Date: | 6-Jul-2022 | Journal: | Scientific reports | Series/Report no.: | Sci Rep;12(1)2022:1-8 | Abstract: | Sepsis has been called the graveyard of pharmaceutical companies due to the numerous failed clinical trials. The lack of tools to monitor the immunological status in sepsis constrains the development of therapies. Here, we evaluated a test based on whole plasma peptidome acquired by MALDI-TOF-mass spectrometer and machine-learning algorithms to discriminate two lipopolysaccharide-(LPS) induced murine models emulating the pro- and anti-inflammatory/immunosuppression environments that can be found during sepsis. The LPS group was inoculated with a single high dose of LPS and the IS group was subjected to increasing doses of LPS, to induce proinflammatory and anti-inflammatory/immunosuppression profiles respectively. The LPS group showed leukopenia and higher levels of cytokines and tissue damage markers, and the IS group showed neutrophilia, lymphopenia and decreased humoral response. Principal component analysis of the plasma peptidomes formed discrete clusters that mostly coincided with the experimental groups. In addition, machine-learning algorithms discriminated the different experimental groups with a sensitivity of 95.7% and specificity of 90.9%. Data reveal the potential of plasma fingerprints analysis by MALDI-TOF-mass spectrometry as a simple, speedy and readily transferrable method for sepsis patient stratification that would contribute to therapeutic decision-making based on their immunological status. |
Description: | Fil: Ledesma, Martín. Laboratorio de Bacteriología, Departamento de Bioquímica Clínica, Hospital de Clínicas "José de San Martín", Facultad de Farmacia y Bioquímica; CABA Fil: Todero, María Florencia. Instituto de Medicina Experimental (IMEX) - CONICET - Academia Nacional de Medicina; CABA Fil: Maceira, Lautaro. Instituto de Medicina Experimental (IMEX) - CONICET - Academia Nacional de Medicina; CABA Fil: Prieto, Mónica. Servicio de Bacteriología Especial. Instituto Nacional de Enfermedades Infecciosas (INEI), ANLIS "Dr. C. G. Malbrán"; CABA Fil: Vay, Carlos. Laboratorio de Bacteriología, Departamento de Bioquímica Clínica, Hospital de Clínicas "José de San Martín", Facultad de Farmacia y Bioquímica; CABA Fil: Galas, Marcelo. Special Program of AMR, Communicable Diseases and Environmental Determinants of Health Department, Pan-American Health Organization; USA Fil: López, Beatriz. Departamento de Bacteriología. INEI, ANLIS "Dr. C. G. Malbrán"; CABA Fil: Yokobori, Noemí. Servicio de Micobacterias INEI, ANLIS "Dr. C. G. Malbrán"; CABA Fil: Rearte, Bárbara. Instituto de Medicina Experimental (IMEX) - CONICET - Academia Nacional de Medicina; CABA |
URI: | http://sgc.anlis.gob.ar/handle/123456789/2554 | DOI: | 10.1038/s41598-022-15792-5 |
Appears in Collections: | Artículos |
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10.1038s41598-022-15792-5.pdf | 1.35 MB | Adobe PDF | View/Open |
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