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Title: | Humoral and cellular immune memory response 12 years following single dose vaccination against hepatitis A in Argentinian children | Authors: | Urueña, A Badano, M N Baré, P González, J V Vicentín, R Calli Flores, Rogelio Cañero-Velasco, M C Fink, S Vizzotti, C |
Keywords: | Virus de la Hepatitis A;Inmunidad;Respuesta de memoria;Vacunación | Issue Date: | 3-Jan-2022 | Journal: | Vaccine | Abstract: | Infants' universal hepatitis A virus (HAV) single-dose vaccination has been highly effective for controlling HAV infection in Argentina, and in other Latin-American countries that adopted that strategy. Although antibodies wane over time, this has not been associated with HAV outbreaks or breakthrough infections, suggesting a relevant role for memory immunity. This study assessed long term humoral and cellular immune memory response after an average of 12 years follow-up of HAV single-dose vaccination. We selected 81 HAV-single dose vaccinated individuals from a 2015 study, including 54 with unprotective (UAL) and 27 with protective antibody levels (PAL) against HAV. Humoral memory response was assessed by measuring anti-HAV antibody titers at admission in both groups, and 30 days after a booster dose in the UAL group. Flow cytometry analysis of peripheral blood mononuclear cell samples stimulated with HAV antigen was performed in 47/81 individuals (21 with PAL, 26 with UAL) to identify activated CD4 + memory T cells or CD8 + memory T cells. The results showed that 48/52 (92%) individuals from UAL group who completed follow up reached protective levels after booster dose. In the PAL group, anti-HAV Abs waned in 2/27 (7%) individuals lacking seroprotection, while in 25/27 (93%) Abs remained >10 mUI/mL. HAV-specific memory CD4 + T cells were detected in 25/47 (53.2%) subjects while HAV-specific memory CD8 + T cells were observed in 16/47 (34.04%) individuals. HAV-specific memory CD4+ and CD8+ T cell responses were detected in 11/21 (52.4%) and in 9/21 (42.9%) subjects with PAL and in 14/26 (53.8%) and in 7/26 (26.9%) individuals with UAL, showing that the presence of memory T-cells was independent of the level or presence of anti-HAV antibodies. Long-term immunity demonstrated in the present work, including or not antibody persistence, suggests that individuals with waned Ab titers may still be protected and supports the single-dose HAV strategy. |
Description: | Fil: Urueña, A. ISALUD University, Centro de Estudios para la Prevención y Control de Enfermedades Transmisibles; Ciudad Autónoma de Buenos Aires, Argentina Fil: Badano, M N. Instituto de Medicina Experimental (IMEX)-CONICET. Academia Nacional de Medicina., Laboratorio de Patogenia de Infecciones Virales; Ciudad Autónoma de Buenos Aires, Argentina Fil: Baré, P. Instituto de Medicina Experimental (IMEX)-CONICET. Academia Nacional de Medicina., Laboratorio de Patogenia de Infecciones Virales; Ciudad Autónoma de Buenos Aires, Argentina Fil: González, J. INEI ANLIS "Dr. C. G. Malbrán", Laboratorio Nacional de Referencia para Hepatitis virales.Departamento Virología; Ciudad Autónoma de Buenos Aires, Argentina Fil: Vicentín, R. Hospital de niños Orlando Alassia; Ciudad de Santa Fe, Santa Fe, Argentina Fil: Calli, R. Dirección de Epidemiología, Ministerio de Salud Pública Provincia de Tucumán; Tucumán, Argentina Fil: Cañero-Velasco, M C. Hospital Eva Perón de la Municipalidad de Merlo; Merlo, Provincia de Buenos Aires, Argentina Fil: Fink, S. Instituto de Medicina Experimental (IMEX)-CONICET. Academia Nacional de Medicina., Laboratorio de Patogenia de Infecciones Virales; Ciudad Autónoma de Buenos Aires, Argentina Fil: Vizzotti, C. ISALUD University, Centro de Estudios para la Prevención y Control de Enfermedades Transmisibles; Ciudad Autónoma de Buenos Aires, Argentina |
URI: | http://sgc.anlis.gob.ar/handle/123456789/2500 | DOI: | 10.1016/j.vaccine.2021.11.037 |
Appears in Collections: | Artículos |
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