Please use this identifier to cite or link to this item: http://sgc.anlis.gob.ar/handle/123456789/2400
Title: One Step Histological Detection and Staining of the PTEN Tumor Suppressor Protein by a Single Strand DNA
Authors: Longinotti, Gloria 
Ybarra, Gabriel 
Vighi, Susana 
Perandones, Claudia 
Montserrat, Javier 
Yakisich, Juan Sebastian 
Grasselli, Mariano 
Radrizzani, Martin 
Keywords: ADN Catalítico;Fosfohidrolasa PTEN;Aptámeros de Nucleótidos;Endometrio
Issue Date: 26-Jan-2021
Journal: Diagnostics (Basel, Switzerland) 
Abstract: 
Antibodies are the most used technological tool in histochemistry. However, even with monoclonal antibodies, their standardization is difficult due to variation of biological systems as well as to variability due to the affinity and amplification of the signal arising from secondary peroxidase detection systems. In this article we combined two synthetic molecules to facilitate the standardization of a detection protocol of protein markers in histological sections. The first molecule was an aptamer, a 50-base single-stranded DNA fragment, which recognizes a PTEN tumor suppressor. The second molecule used was also another single stranded 18-base aptamer DNA fragment, which forms a quadruplex structure guanine box. This G-quadruplex recognizes and attaches a molecule of hemin, increasing the catalytic capacity for the hydrogen peroxide. Our results show how the correct structural design of DNA combining an aptamer together with the peroxidase-like DNAzyme allows to detect proteins in histological sections. This tool offers the standardization of the detection of prognostic markers in cancer, in quality and quantity, due to its synthetic nature and its 1:1 antigen:enzyme ratio. This is the first time that reproducible results have been presented in histological sections staining a cancer marker using a single-stranded DNA molecule with dual function.
Description: 
Fil. Longinotti, Gloria. Nanomateriales Funcionales, INTI-Micro y Nanotecnologías, Instituto Nacional de Tecnología Industrial (INTI), Av. Gral. Paz 5445, San Martín B1650WAB, Argentina.

Fil. Ybarra, Gabriel. Nanomateriales Funcionales, INTI-Micro y Nanotecnologías, Instituto Nacional de Tecnología Industrial (INTI), Av. Gral. Paz 5445, San Martín B1650WAB, Argentina.

Fil. Vighi, Susana. Centro de Anatomía Patológica, Ciudad de la Paz 353, Buenos Aires C1426AGE, Argentina.

Fil. Perandones, Claudia. ANLIS Dr.C.G.Malbrán. Unidad Ejecutora de Formación y Educación Superior; Argentina.

Fil. Montserrat, Javier. Instituto de Ciencias, Universidad Nacional de General Sarmiento, JM Gutiérrez 1150, Los Polvorines B1613GSX, Argentina.

Fil. Yakisich, Juan Sebastián. Departamento de Ciencias Farmacéuticas, Facultad de Farmacia, Universidad de Hampton, Hampton, VA 23693, EE. UU.

Fil. Grasselli, Mariano. Laboratorio de Materiales Biotecnológicos (LaMaBio), Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, GBE yB, Grupo Vinculado IMBICE-CONICET, Roque Sáenz Peña 352, Buenos Aires B1876BXDl, Argentina.

Fil. Radrizzani, Martín. Laboratorio de Neurología y Citogenética Molecular, (CESyMA), Escuela de Ciencia y Técnica, Universidad Nacional de San Martín, Av. Gral. Paz 5445, San Martín B1650WAB, Argentina.
URI: http://sgc.anlis.gob.ar/handle/123456789/2400
ISSN: 2075-4418
DOI: 10.3390/diagnostics11020171
Appears in Collections:Publicaciones UEFES

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