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Title: | Identification of Two New Sequences of Flagellin-Encoding Gene in Escherichia coli Using PCR and Sequencing-Based Methods | Authors: | Rogé, Ariel Diego Celi Castillo, Ana Beatriz van der Ploeg, Claudia Andrea Bordagorría, Ximena Luciana Padín, Valeria M Bruno, Susana Beatriz |
Keywords: | Adhesinas de Escherichia coli;Análisis de Secuencia;Activación Transcripcional;Antígeno B7-H1 | Issue Date: | 3-Sep-2021 | Journal: | Foodborne pathogens and disease | Abstract: | Escherichia coli has traditionally been serotyped using antisera against the O and H antigens. However, a proportion of E. coli isolates are nonmotile and, in addition, some isolates do not react with the currently available H-typing sera. Alternative molecular methods have been developed based on the detection of genes encoding for H antigens. In this study, we studied 13 serologically nontypable H antigen E. coli strains using polymerase chain reaction (PCR) and sequencing-based methods. We found two new sequences of flagellin-encoding gene, for each of which a specific antiserum was produced to confirm their expression. Sequencing of the flagellin gene offers a rapid determination of E. coli H antigens and could be used to detect potential novel flagellar antigens. |
Description: | Fil. Rogé, Ariel Diego. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos. Servicio antígenos y antisueros; Argentina. Fil. Celi Castillo, Ana Beatriz. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos. Servicio antígenos y antisueros; Argentina. Fil. van der Ploeg, Claudia Andrea. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos. Servicio antígenos y antisueros; Argentina. Fil. Bordagorría, Ximena Luciana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos. Servicio antígenos y antisueros; Argentina. Fil. Padín, Valeria M. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos. Servicio antígenos y antisueros; Argentina. Fil. Bruno , Susana Beatriz. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos. Servicio antígenos y antisueros; Argentina. |
URI: | http://sgc.anlis.gob.ar/handle/123456789/2391 | DOI: | 10.1089/fpd.2021.0033 |
Appears in Collections: | Publicaciones INPB |
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