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Title: | Neutralizing activity of Sputnik V vaccine sera against SARS-CoV-2 variants | Authors: | Ikegame, Satoshi Siddiquey, Mohammed N A Hung, Chuan-Tien Haas, Griffin Brambilla, Luca Oguntuyo, Kasopefoluwa Y Kowdle, Shreyas Chiu, Hsin-Ping Stevens, Christian S Vilardo, Ariel Esteban Edelstein, Alexis Perandones, Claudia Kamil, Jeremy P Lee, Benhur |
Keywords: | Compartimentos de Replicación Viral;Anticuerpos Anti-HTLV-I;Anticuerpos Neutralizantes;Vacunas contra la COVID-19;Células HEK293 | Issue Date: | Jul-2021 | Journal: | Nature communications | Abstract: | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected at least 180 million people since its identification as the cause of the current COVID-19 pandemic. The rapid pace of vaccine development has resulted in multiple vaccines already in use worldwide. The contemporaneous emergence of SARS-CoV-2 'variants of concern' (VOC) across diverse geographic locales underscores the need to monitor the efficacy of vaccines being administered globally. All WHO designated VOC carry spike (S) polymorphisms thought to enable escape from neutralizing antibodies. Here, we characterize the neutralizing activity of post-Sputnik V vaccination sera against the ensemble of S mutations present in alpha (B.1.1.7) and beta (B.1.351) VOC. Using de novo generated replication-competent vesicular stomatitis virus expressing various SARS-CoV-2-S in place of VSV-G (rcVSV-CoV2-S), coupled with a clonal 293T-ACE2 + TMPRSS2 + cell line optimized for highly efficient S-mediated infection, we determine that only 1 out of 12 post-vaccination serum samples shows effective neutralization (IC90) of rcVSV-CoV2-S: B.1.351 at full serum strength. The same set of sera efficiently neutralize S from B.1.1.7 and exhibit only moderately reduced activity against S carrying the E484K substitution alone. Taken together, our data suggest that control of some emergent SARS-CoV-2 variants may benefit from updated vaccines. |
Description: | Fil. Ikegame, Satoshi. Departamento de Microbiología de la Escuela de Medicina Icahn en Mount Sinai, Nueva York, NY, EE. UU. Fil. NA Siddiquey, Mohammed. Departamento de Microbiología e Inmunología, Universidad Estatal de Louisiana Health Shreveport, Shreveport, LA, EE. UU. Fil. Chuan-Tien Hung. Departamento de Microbiología de la Escuela de Medicina Icahn en Mount Sinai, Nueva York, NY, EE. UU. Fil. Griffin Haas. Departamento de Microbiología de la Escuela de Medicina Icahn en Mount Sinai, Nueva York, NY, EE. UU. Fil. Brambilla, Luca. Departamento de Microbiología de la Escuela de Medicina Icahn en Mount Sinai, Nueva York, NY, EE. UU. Fil. Kasopefoluwa Y Oguntuyo. Departamento de Microbiología de la Escuela de Medicina Icahn en Mount Sinai, Nueva York, NY, EE. UU. Fil. Shreyas Kowdle, Departamento de Microbiología de la Escuela de Medicina Icahn en Mount Sinai, Nueva York, NY, EE. UU. Fil. Hsin-Ping Chiu. Departamento de Microbiología de la Escuela de Medicina Icahn en Mount Sinai, Nueva York, NY, EE. UU. Fil. Stevens, Christian S. Departamento de Microbiología de la Escuela de Medicina Icahn en Mount Sinai, Nueva York, NY, EE. UU. Fil. Vilardo, Ariel Esteban. Administración Nacional de Laboratorios e Institutos de Salud de Argentina (ANLIS) Dr. Carlos G. Malbrán, Buenos Aires, Argentina. Fil. Edelstein. Alexis. Administración Nacional de Laboratorios e Institutos de Salud de Argentina (ANLIS) Dr. Carlos G. Malbrán, Buenos Aires, Argentina. Fil. Perandones, Claudia. Administración Nacional de Laboratorios e Institutos de Salud de Argentina (ANLIS) Dr. Carlos G. Malbrán, Buenos Aires, Argentina. Fil. Kamil, Jeremy P. Departamento de Microbiología e Inmunología, Universidad Estatal de Louisiana Health Shreveport, Shreveport, LA, EE. UU. Fil. Benhur Lee. Departamento de Microbiología de la Escuela de Medicina Icahn en Mount Sinai, Nueva York, NY, EE. UU. |
URI: | http://sgc.anlis.gob.ar/handle/123456789/2376 | DOI: | 10.1038/s41467-021-24909-9 |
Appears in Collections: | Publicaciones |
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41467_2021_Article_24909 (1).pdf | artículo en inglés | 1.8 MB | Adobe PDF | View/Open |
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