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Title: | Genetic characterization of Vibrio cholerae isolates from Argentina by V. cholerae repeated sequences-polymerase chain reaction | Authors: | Castañeda, Nancy Claudia Pichel, Mariana Orman, Betina E. Binsztein, Norma Roy, Paul H Centrón, Daniela |
Keywords: | Argentina;Cólera;ADN Bacteriano;Microbiología Ambiental;Humanos;Reacción en Cadena de la Polimerasa;Secuencias Repetitivas de Ácidos Nucleicos;Vibrio cholerae O1;Vibrio cholerae no O1;Técnicas de Tipificación Bacteriana;Brotes de Enfermedades | Issue Date: | Nov-2005 | Publisher: | Elsevier | Project: | datasets | Journal: | Diagnostic microbiology and infectious disease | Abstract: | We have developed a novel typing method based on Vibrio cholerae repeat sequences (VCR) using primers directed out of the VCR sequences. To evaluate the VCR-polymerase chain reaction (PCR) as a typing system, 2 categories, efficacy and efficiency, were analyzed in 69 strains of human and environmental V. cholerae O1 toxigenic and nontoxigenic, and non-O1 strains isolated since 1992-2000 from Argentina. The discriminatory power (0.91), stability (0.95), reproducibility (1), typeability (1), rapidity, accessibility, as well ease of use, indicated that the VCR-PCR method provides an alternative useful tool for molecular epidemiology of V. cholerae. The VCR-PCR of V. cholerae isolates showed 29 patterns, of which pattern 1 represented 68% of the V. cholerae O1 isolates, supporting the hypothesis that a clone with epidemic behavior was responsible for the epidemic in Latin America. These results showed a good correlation and a better epidemiologic analysis when the results were compared in parallel with repetitive extragenic palindromic sequences-PCR. In conclusion, VCR-PCR showed excellent performance as a typing method for cholera surveillance programs. |
Description: | Fil: Castañeda, Nancy Claudia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología; Argentina. Fil: Pichel, Mariana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología; Argentina. Fil: Orman, Betina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología, Inmunología y Parasitología; Argentina. Fil: Binsztein, Norma. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología; Argentina. Fil: Roy, Paul H. Centre Hospitalier Universitaire de Québec. Centre de Recherche en lnfectiologie; Canadá. Fil: Centrón, Daniela. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología, Inmunología y Parasitología; Argentina. |
URI: | http://sgc.anlis.gob.ar/handle/123456789/1984 | ISSN: | 0732-8893 | DOI: | 10.1016/j.diagmicrobio.2005.05.008 | Rights: | Open Access |
Appears in Collections: | Publicaciones INEI |
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