Please use this identifier to cite or link to this item:
http://sgc.anlis.gob.ar/handle/123456789/1984
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Castañeda, Nancy Claudia | es |
dc.contributor.author | Pichel, Mariana | es |
dc.contributor.author | Orman, Betina E. | es |
dc.contributor.author | Binsztein, Norma | es |
dc.contributor.author | Roy, Paul H | es |
dc.contributor.author | Centrón, Daniela | es |
dc.date.accessioned | 2020-12-23T15:39:42Z | - |
dc.date.available | 2020-12-23T15:39:42Z | - |
dc.date.issued | 2005-11 | - |
dc.identifier.issn | 0732-8893 | - |
dc.identifier.uri | http://sgc.anlis.gob.ar/handle/123456789/1984 | - |
dc.description | Fil: Castañeda, Nancy Claudia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología; Argentina. | es |
dc.description | Fil: Pichel, Mariana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología; Argentina. | es |
dc.description | Fil: Orman, Betina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología, Inmunología y Parasitología; Argentina. | es |
dc.description | Fil: Binsztein, Norma. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología; Argentina. | es |
dc.description | Fil: Roy, Paul H. Centre Hospitalier Universitaire de Québec. Centre de Recherche en lnfectiologie; Canadá. | es |
dc.description | Fil: Centrón, Daniela. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología, Inmunología y Parasitología; Argentina. | es |
dc.description.abstract | We have developed a novel typing method based on Vibrio cholerae repeat sequences (VCR) using primers directed out of the VCR sequences. To evaluate the VCR-polymerase chain reaction (PCR) as a typing system, 2 categories, efficacy and efficiency, were analyzed in 69 strains of human and environmental V. cholerae O1 toxigenic and nontoxigenic, and non-O1 strains isolated since 1992-2000 from Argentina. The discriminatory power (0.91), stability (0.95), reproducibility (1), typeability (1), rapidity, accessibility, as well ease of use, indicated that the VCR-PCR method provides an alternative useful tool for molecular epidemiology of V. cholerae. The VCR-PCR of V. cholerae isolates showed 29 patterns, of which pattern 1 represented 68% of the V. cholerae O1 isolates, supporting the hypothesis that a clone with epidemic behavior was responsible for the epidemic in Latin America. These results showed a good correlation and a better epidemiologic analysis when the results were compared in parallel with repetitive extragenic palindromic sequences-PCR. In conclusion, VCR-PCR showed excellent performance as a typing method for cholera surveillance programs. | es |
dc.language.iso | en | es |
dc.publisher | Elsevier | es |
dc.relation | datasets | - |
dc.relation.ispartof | Diagnostic microbiology and infectious disease | es |
dc.rights | Open Access | - |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | - |
dc.source | Diagnostic Microbiology and Infectious Disease 2005; 53(3):175-83 | - |
dc.subject | Argentina | es |
dc.subject | Cólera | es |
dc.subject | ADN Bacteriano | es |
dc.subject | Microbiología Ambiental | es |
dc.subject | Humanos | es |
dc.subject | Reacción en Cadena de la Polimerasa | es |
dc.subject | Secuencias Repetitivas de Ácidos Nucleicos | es |
dc.subject | Vibrio cholerae O1 | es |
dc.subject | Vibrio cholerae no O1 | es |
dc.subject | Técnicas de Tipificación Bacteriana | es |
dc.subject | Brotes de Enfermedades | es |
dc.title | Genetic characterization of Vibrio cholerae isolates from Argentina by V. cholerae repeated sequences-polymerase chain reaction | es |
dc.type | Artículo | es |
dc.rights.license | Creative Commons Attribution 4.0 International License | - |
dc.identifier.doi | 10.1016/j.diagmicrobio.2005.05.008 | - |
anlis.essnrd | 1 | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.openairetype | Artículo | - |
item.fulltext | No Fulltext | - |
item.languageiso639-1 | en | - |
crisitem.author.dept | Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS) | - |
crisitem.author.dept | Instituto Nacional de Enfermedades Infecciosas (INEI) | - |
crisitem.author.dept | Departamento de Bacteriología | - |
crisitem.author.dept | Servicio Enterobacterias | - |
crisitem.author.parentorg | Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS) | - |
crisitem.author.parentorg | Instituto Nacional de Enfermedades Infecciosas (INEI) | - |
crisitem.author.parentorg | Departamento de Bacteriología | - |
Appears in Collections: | Publicaciones INEI |
This item is licensed under a Creative Commons License