Biochemical Characterization of Metallo-β-Lactamase VIM-11 from a Pseudomonas aeruginosa Clinical Strain

Abstract

A detailed biochemical characterization of the Pseudomonas aeruginosa VIM-11 metallo- -lactamase (M L) is reported. The only substitution differentiating VIM-11 from VIM-2 (N165S) promoted a slightly improved catalytic efficiency of the former on 3 out of 12 substrates, notably the bulky cephalosporins. Thus, M Lmediated resistance also may be modulated by remote mutations.