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Title: | Simplified Protocol for Carba NP Test for Enhanced Detection of Carbapenemase Producers Directly from Bacterial Cultures | Authors: | Pasteran, Fernando Tijet, Nathalie Melano, Roberto Corso, Alejandra |
Keywords: | Proteínas Bacterianas;Técnicas Bacteriológicas;beta-Lactamasas | Issue Date: | Dec-2015 | Publisher: | American Society for Microbiology | Journal: | Journal of clinical microbiology | Abstract: | We compared carbapenemase detection among 266 Gram-negative bacilli (161 carbapenemase producers) using the Carba NP tests issued by the CLSI (CNPt-CLSI) and a novel protocol (CNPt-direct) designed for carbapenemase detection direct from bacterial cultures (instead of bacterial extracts required by the CLSI tests). The specificities were comparable (100%), but the CNPt-direct was more sensitive (98% versus 84%). The CNPt-direct was easier to perform due to the direct use of colonies and offered a more robust detection of carbapenemase producers. |
Description: | Fil: Pasteran, Fernando. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina. Fil: Tijet, Nathalie Public Health Ontario Laboratories (PHOL); Canada. Fil: Melano, Roberto. Public Health Ontario Laboratories (PHOL, Canada Department of Laboratory Medicine and Pathobiology, University of Toronto; Canada. Fil: Corso, Alejandra. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina. |
URI: | http://sgc.anlis.gob.ar/handle/123456789/1799 | ISSN: | 1098-660X | DOI: | 10.1128/JCM.02032-15 | Rights: | Open Access Creative Commons Attribution 4.0 International License |
Appears in Collections: | Publicaciones INEI |
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10.1128_JCM.02032-15.pdf | Artículo en inglés | 326.34 kB | Adobe PDF | View/Open |
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