Please use this identifier to cite or link to this item: http://sgc.anlis.gob.ar/handle/123456789/1750
Title: Characterization and molecular subtyping of Shiga toxin-producing Escherichia coli strains in provincial abattoirs from the Province of Buenos Aires, Argentina, during 2016-2018
Authors: Costa, Magdalena 
Londero, Alejandra 
Brusa, Victoria 
Galli, Lucía 
van der Ploeg, Claudia Andrea 
Rogé, Ariel 
Leotta, Gerardo A 
Keywords: Mataderos;Toxina Shiga;Escherichia coli;Argentina
Issue Date: Oct-2020
Publisher: Elsevier
Journal: Preventive veterinary medicine 
Abstract: 
We characterized Shiga toxin-producing Escherichia coli (STEC) O157 (n = 20) and non-O157 (n = 68) isolated from carcasses (n = 54), the environment (n = 20), head meat (n = 3) and viscera washing and chilling water (n = 11) in provincial abattoirs before and after implementing improvement actions. The strains were tested for eae, saa, ehxA and fliCH7 genes. Variants stx1 and stx2 were also determined. Pulsed-field gel electrophoresis (PFGE) was carried out with restriction enzymes XbaI and BlnI. All twenty O157 STEC strains [H7; H21; HNM] carried genes rfbO157 and ehxA; 90.0 % were positive for eae and 15.0 % were negative for fliCH7 and positive for saa. Results of PFGE showed 17 XbaI patterns, of which 14 were unique and three formed clusters. From the 68 non-O157 STEC strains, 66.2 %, 55.9 % and 2.9 % were positive for ehxA, saa and eae genes, respectively. Fifty-three XbaI patterns were obtained (49 unique and four forming clusters). Cross-contamination between products and between the environment and products was confirmed in all abattoirs. While the proposed improvements reduced the risk of contamination, Good Hygiene Practices and Good Manufacturing Practices should be implemented in provincial abattoirs, stressing the importance of having a uniform national food safety standard.
Description: 
Fil: Costa, Magdalena. GEVET - Instituto de Genética Veterinaria "Ing. Fernando N. Dulout" (UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias; Argentina.

Fil: Londero, Alejandra. GEVET - Instituto de Genética Veterinaria "Ing. Fernando N. Dulout" (UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias; Argentina.

Fil: Brusa, Victoria. GEVET - Instituto de Genética Veterinaria "Ing. Fernando N. Dulout" (UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias; Argentina.

Fil: Galli, Lucía.GEVET - Instituto de Genética Veterinaria "Ing. Fernando N. Dulout" (UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias; Argentina.

Fil: Van Der Ploeg, Claudia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos. Servicio Antígenos y Antisueros; Argentina.

Fil: Roge, Ariel. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos. Servicio Antígenos y Antisueros; Argentina.

Fil: Leotta, Gerardo A. GEVET - Instituto de Genética Veterinaria "Ing. Fernando N. Dulout" (UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias; Argentina.
URI: http://sgc.anlis.gob.ar/handle/123456789/1750
ISSN: 0167-5877
DOI: 10.1016/j.prevetmed.2020.105133
Rights: Closed Access
Appears in Collections:Publicaciones INPB

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