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Title: Comparison of DNA extraction methods and real-time PCR assays for the molecular diagnostics of chronic Chagas disease
Authors: Silgado, Aroa 
Moure, Zaira 
de Oliveira, Maykon T 
Serre-Delcor, Núria 
Salvador, Fernando 
Oliveira, Inés 
Molina, Israel 
Pumarola, Tomàs 
Ramirez, Juan C. 
Sulleiro, Elena 
Keywords: Enfermedad de Chagas;Extracción de ADN;Trypanosoma cruzi;Ensayo Clínico;Diagnóstico Molecular
Issue Date: Aug-2020
Journal: Future microbiology 
Aim: This work aimed to compare the sensitivity of four protocols for the detection of Trypanosoma cruzi DNA in 98 blood samples from chronic Chagas disease patients. Materials & methods: Two DNA extraction (automated and manual) methods and two T. cruzi satellite DNA qPCRs (with a recent design and the usually used set of primers) were analyzed. Results: Both DNA extraction methods and qPCR assays tested in this work gave comparable qualitative results, although the lowest Ct values were obtained when samples were analyzed using the new set of primers for T. cruzi satellite DNA. Conclusion: Our results encourage the implementation of automated DNA extraction systems and the new T. cruzi qPCR for the molecular diagnostics and treatment response monitoring of chronic Chagas disease patients.
Fil: Ramírez, Juan C. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología DR: Mario Fatala Chaben; Argentina.
DOI: 10.2217/fmb-2020-0023
Appears in Collections:Publicaciones INP

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