Please use this identifier to cite or link to this item: http://sgc.anlis.gob.ar/handle/123456789/1511
Title: Immunodiagnosis of fasciolosis using recombinant procathepsin L cystein proteinase
Authors: Carnevale, Silvana 
Rodríguez, M I 
Guarnera, E A 
Carmona, C 
Tanos, Tamara 
Angel, Sergio O. 
Issue Date: 2001
Journal: Diagnostic microbiology and infectious disease 
Abstract: Cathepsin L1, a cysteine protease secreted by the gastrodermis of juvenile and adult Fasciola hepatica, was expressed in Escherichia coli as a fusion protein containing the proregion, supplied with six histidyl residues at the N-terminal end (rproCL1). In this study we tested its potential as antigen for the serologic diagnosis of F. hepatica infections by enzyme-linked immunosorbent assay (ELISA). The analyzed human sera included 16 positive samples, 99 negative controls and 111 from individuals affected by other parasitic and non parasitic diseases. The sensitivity and specificity of the rproCL1-ELISA were 100%. We also assessed the ability to detect antibodies in sera from 10 experimentally infected sheep, obtaining preliminary results that shown a response since the third week post infection in all the studied animals. Therefore, the recombinant rproCL1-based ELISA could be a standardized test for the accurate diagnosis of fasciolosis.
URI: http://sgc.anlis.gob.ar/handle/123456789/1511
ISSN: 0732-8893
DOI: 10.1016/s0732-8893(01)00288-7
Appears in Collections:Publicaciones INP

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