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Title: | Diagnosis of Enterocytozoon bieneusi by PCR in stool samples eluted from filter paper disks | Authors: | Carnevale, Silvana Velásquez, Jorge Néstor Labbé, J H Chertcoff, A Cabrera, Marta Rodriguez, Marcelo |
Issue Date: | May-2000 | Journal: | Clinical and diagnostic laboratory immunology | Abstract: | We report a PCR-based assay for the detection of Enterocytozoon bieneusi. We extracted DNA from feces which had been applied to filter paper disks and evaluated four preserving solutions. Infected specimens were identified by electrophoresis of amplicons from concentrated formalin-fixed samples and unconcentrated fresh feces. Our findings demonstrate that this methodology is effective for sample collection, mailing, and diagnosis of this pathogen. |
URI: | http://sgc.anlis.gob.ar/handle/123456789/1505 | ISSN: | 1071-412X | DOI: | 10.1128/cdli.7.3.504-506.2000 |
Appears in Collections: | Publicaciones INP |
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File | Description | Size | Format | |
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cd000504.pdf | Artículo en inglés | 128.7 kB | Adobe PDF | View/Open |
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