Please use this identifier to cite or link to this item: http://sgc.anlis.gov.ar/handle/123456789/1391
Title: Enzyme-linked immunosorbent assay to diagnose human leptospirosis: a meta-analysis of the published literature
Authors: Signorini, M L 
Lottersberger, Javier 
Tarabla, H D 
Vanasco, Bibiana N. 
Issue Date: Jan-2013
Journal: Epidemiology and infection 
Abstract: 
We report an evaluation of the accuracy of ELISA for the detection of Leptospira-specific antibodies in humans. Eighty-eight studies published in 35 articles met all inclusion criteria and were submitted to meta-analysis. Pooled sensitivity and specificity were 0·779 (95% CI 0·770-0·789) and 0·913 (95% CI 0·908-0·917), respectively, and the area under the curve was 0·964. Heterogeneity across studies was statistically significant, but none of the sources of heterogeneity (disease stage, antigen used, antibody detected) could fully explain this finding. Although the convalescent stage of disease was significantly associated with higher diagnostic accuracy, IgM ELISA was the best choice, regardless of the stage of disease. Negative ELISAs (IgG or IgM) applied in the acute phase do not rule out leptospirosis due to the possibility of false-negative results. In this case it is advisable to request a second blood sample or to apply a direct method for leptospiral DNA.
URI: http://sgc.anlis.gob.ar/handle/123456789/1391
DOI: 10.1017/S0950268812001951
Appears in Collections:Publicaciones INER

Show full item record

Page view(s)

4
checked on Jul 4, 2020

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.