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Title: | Bedaquiline and linezolid MIC distributions and epidemiological cut-off values for Mycobacterium tuberculosis in the Latin American region | Authors: | López, Beatriz Siqueira de Oliveira, Rosangela Pinhata, Juliana M W Chimara, Erica Pacheco Ascencio, Edson Puyén Guerra, Zully M Wainmayer, Ingrid Simboli, Norberto Del Granado, Mirtha Palomino, Juan Carlos Ritacco, Viviana Martin, Anandi |
Keywords: | Tuberculosis Resistente a Múltiples Medicamentos;Antituberculosos;Mycobacterium tuberculosis | Issue Date: | 1-Feb-2019 | Journal: | The Journal of antimicrobial chemotherapy | Abstract: | Objectives: To describe the distributions of bedaquiline and linezolid MIC values for the Mycobacterium tuberculosis WT population and to define the corresponding epidemiological cut-offs (ECOFFs) in three Latin American countries. Methods: MICs of bedaquiline and linezolid were determined by the resazurin microtitre assay (REMA). In phase 1, interlaboratory reproducibility was assessed using a panel of 10 fully susceptible M. tuberculosis strains. Phase 2 involved MIC determination for 248 clinical isolates from Argentina (n = 58), Brazil (n = 100) and Peru (n = 90) from patients who were treatment-naive for bedaquiline and linezolid. We then determined the ECOFFs for bedaquiline and linezolid by the eyeball method and the ECOFFinder statistical calculator. Results: Phase 1: REMA MIC values in the three sites were either identical to each other or differed by one 2-fold dilution from the consensus value with the exception of a single value. Phase 2: the bedaquiline MIC range was 0.0039-0.25 mg/L for pan-susceptible and drug-resistant isolates combined. The linezolid MIC range was 0.062-0.5 mg/L for pan-susceptible isolates and 0.031-4 mg/L for drug-resistant isolates. ECOFFs were 0.125 mg/L for bedaquiline and 0.50 mg/L for linezolid. Conclusions: REMA is reproducible and robust for the determination of bedaquiline and linezolid MIC distributions and ECOFF values when applied in laboratories of medium/low-resource countries. We suggest that WT MIC distributions for both drugs should be used as a monitoring tool to control the possible rapid emergence of resistance. |
Description: | Fil: López, Beatriz. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriologia; Argentina. Fil: Siqueira de Oliveira, Rosangela. Instituto Adolfo Lutz Sao Paulo. Centro de Bacteriología. Nucleo de Tuberculose e Micobacterioses; Brasil. Fil: Pinhata, Juliana M. W. Instituto Adolfo Lutz Sao Paulo. Centro de Bacteriología. Nucleo de Tuberculose e Micobacterioses; Brasil. Fil: Chimara, Erica. Instituto Adolfo Lutz Sao Paulo. Centro de Bacteriología. Nucleo de Tuberculose e Micobacterioses; Brasil. Fil: Pacheco Ascencio, Edson. Instituto Nacional de Salud. Laboratorio de Referencia Nacional de Micobacterias; Lima, Perú. Fil: Puyén Guerra, Zully M. Instituto Nacional de Salud. Laboratorio de Referencia Nacional de Micobacterias; Lima, Perú. Fil: Wainmayer, Ingrid. ANLIS Dr.C.G.Malbrán. Insituto Nacional de Enfermededades Infecciosas. Servicio de Micobacterias; Argentina. Fil: Simboli, Norberto. ANLIS Dr.C.G.Malbrán. Insituto Nacional de Enfermededades Infecciosas. Servicio de Micobacterias; Argentina. Fil: Del Granado, Mirtha. Pan American Health Organization; Estados Unidos. Fil: Palomino, Juan Carlos. Ghent University. Department of Biochemistry and Microbiology. Faculty of sciences; Belgica. Fil: Ritacco, Viviana. ANLIS Dr.C.G.Malbrán. Insituto Nacional de Enfermededades Infecciosas. Servicio de Micobacterias; Argentina. Fil: Martín, Anandi. Université catholique de Louvain. Institute of Experimental and Clinical Research. Laboratory of Medical Microbiology; Belgica. |
URI: | http://sgc.anlis.gob.ar/handle/123456789/1333 | DOI: | 10.1093/jac/dky414 |
Appears in Collections: | Publicaciones INEI |
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