Please use this identifier to cite or link to this item:
http://sgc.anlis.gob.ar/handle/123456789/467
DC Field | Value | Language |
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dc.contributor.author | Cafferata, Eduardo | es |
dc.contributor.author | González Guerrico, Anatilde M. | es |
dc.contributor.author | Pivetta, Omar H. | es |
dc.contributor.author | Santa-Coloma, Tomás A. | es |
dc.date.accessioned | 2013-04-03T01:09:41Z | - |
dc.date.available | 2013-04-03T01:09:41Z | - |
dc.date.issued | 2001-05-04 | - |
dc.identifier.uri | http://sgc.anlis.gob.ar/handle/123456789/467 | - |
dc.description | Fil: Cafferata, Eduardo. ANLIS Dr.C.G.Malbrán. Centro Nacional de Genética Médica Dr. Eduardo Castilla. Departamento de Genética Experimental; Argentina. | es |
dc.description | Fil: González Guerrico, Anatilde M. ANLIS Dr.C.G.Malbrán. Centro Nacional de Genética Médica Dr. Eduardo Castilla. Departamento de Genética Experimental; Argentina. | es |
dc.description | Fil: Pivetta, Omar H. ANLIS Dr.C.G.Malbrán. Centro Nacional de Genética Médica Dr. Eduardo Castilla. Departamento de Genética Experimental; Argentina. | es |
dc.description | Fil: Santa-Coloma, Tomás A. ANLIS Dr.C.G.Malbrán. Centro Nacional de Genética Médica Dr. Eduardo Castilla. Departamento de Genética Experimental; Argentina. | es |
dc.description.abstract | Interleukin-1 beta (IL-1 beta) regulates the levels of cystic fibrosis transmembrane conductance regulator (CFTR) mRNA and protein in the T84 human carcinoma cell line. Here, we studied the role of the transcription factor NF-kappaB in this regulation. Initially, T84 cells were pretreated with the NF-kappaB inhibitor pyrrolidine dithiocarbamate, Cells were then stimulated with IL-1 beta, and CFTR mRNA levels were determined after 4 h by Northern blot analysis. As a result of PDTC treatment, IL-1 beta stimulation of CFTR mRNA was blocked, On the other hand, daunorubicin, an NF-kappaB activator, increased the steady-state levels of CFTR mRNA, Furthermore, after treatment with IL-1 beta for 1 h, cytoplasmic I kappaB alpha degradation occurred simultaneously with translocation of p65 into the nucleus, The T84 cells were also transduced with an adenoviral vector expressing a dominant negative form of I kappaB alpha, which prevents I kappaB alpha phosphorylation and the subsequent nuclear translocation of NF-kappaB, After viral transduction, the cells were stimulated with IL-1 beta for 4 h, and CFTR mRNA levels were measured by Northern blot analysis. The stimulation of CFTR, induced by IL-1 beta, was also blocked in the presence of the dominant negative mutant. These results indicate that NF-kappaB is involved in the pathway by which IL-1 beta regulates CFTR. | es |
dc.language.iso | en | es |
dc.relation.ispartof | The Journal of biological chemistry | es |
dc.rights | Open Access | en_US |
dc.rights | Creative Commons Attribution 4.0 International License | - |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | - |
dc.source | Journal of Biological Chemistry 2001;276(18):15441-15444 | en_US |
dc.subject | Fibrosis Quística | es |
dc.subject | Interleucina-1beta | es |
dc.subject | Regulador de Conductancia de Transmembrana de Fibrosis Quística | es |
dc.subject | Ensayo de Cambio de Movilidad Electroforética | es |
dc.title | NF-kappa B activation is involved in regulation of cystic fibrosis transmembrane conductance regulator (CFTR) by interleukin-1 beta | es |
dc.type | Artículo | es |
dc.identifier.doi | 10.1074/jbc.M010061200 | - |
anlis.essnrd | 1 | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.openairetype | Artículo | - |
item.fulltext | With Fulltext | - |
item.languageiso639-1 | en | - |
Appears in Collections: | Preproducción Publicaciones CeNaGeM |
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TheJournalofbiologicalchemistry_2001_276_18_p15441-p15444.pdf | 383.19 kB | Adobe PDF | View/Open |
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