Por favor, use este identificador para citar o enlazar este ítem: http://sgc.anlis.gob.ar/handle/123456789/404
Título : Molecular epidemiology of echovirus 30: temporal circulation and prevalence of single lineages
Autor : Palacios, Gustavo 
Casas, Inmaculada 
Cisterna, Daniel 
Trallero, G. 
Tenorio, Antonio 
Freire, María Cecilia 
Palabras clave : Epidemiología Molecular;Infecciones por Echovirus;Prevalencia
Fecha de publicación : may-2002
Resumen : 
Echovirus 30 (EV30) is one of the most frequently isolated EVs, causing extensive outbreaks of EV30 aseptic meningitis in temperate climates. EV30 is antigenically heterogeneous, and three major antigenic groups have been defined, although the basis for the antigenic differences is unknown. A reverse transcription-nested PCR which amplifies the 3'-terminal region of the VP1 gene directly from clinical samples was selected for studying EV30 molecular epidemiology, since the major antigenic sites in this region reflect the serotypic pattern of this virus. The different previous approaches to the genetic classification of EV30 were analyzed. A complete study of the EV30 strains was performed by analyzing the sequences from the 112 EV30 strains amplified in this work and the complete set of EV30 strains previously published. A total of 318 strains of EV30 were divided into two broad genotypes (I and II). This classification was supported by the phylogenetic trees obtained from amino acid sequences, and it correlated with the antigenic heterogeneity of the reference strains described in earlier studies. The genotypes could be further divided into subgroups, and these subgroups could be divided into lineages based on their nucleotide distances and levels of bootstrapping. On the other hand, the subgroups and lineages did not result in the same correlation between amino acid and nucleotide differentiation. The molecular epidemiology of EV30 can be compared to influenza virus epidemiology, where prevailing lineages displace the less established lineages on the basis of immune escape. This pattern of evolution is clearly different from that of other entero-viruses. A single lineage at a time appears to be circulating worldwide. This behavior may be related to the epidemic activity of EV30.
Descripción : 
Fil: Palacios, Gustavo. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología. Servicio de Neurovirosis; Argentina.

Fil: Casas, Inmaculada. Instituto de Salud Carlos III. Centro Nacional de Microbiología. Services of Diagnostic Microbiology and Virology; Argentina.

Fil: Cisterna, Daniel. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología. Servicio de Neurovirosis; Argentina.

Fil: Trallero, G. Instituto de Salud Carlos III. Centro Nacional de Microbiología. Services of Diagnostic Microbiology and Virology; Argentina.

Fil: Tenorio, Antonio. Instituto de Salud Carlos III. Centro Nacional de Microbiología. Services of Diagnostic Microbiology and Virology; Argentina.

Fil: Freire, María Cecilia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología. Servicio de Neurovirosis; Argentina.
URI : http://sgc.anlis.gob.ar/handle/123456789/404
ISSN : 0022-538X
DOI: 10.1128/JVI.76.10.4940–4949.2002
Derechos: Open Access
Creative Commons Attribution License 4.0
Aparece en las colecciones: snrd
Publicaciones INEI

Ficheros en este ítem:
Fichero Descripción Tamaño Formato
JournalofVirology_2002_76_10_p4940-p4949.pdf1.92 MBAdobe PDFVisualizar/Abrir
Mostrar el registro Dublin Core completo del ítem

Visualizaciones de página(s)

53
comprobado en 28-mar-2024

Descarga(s)

3
comprobado en 28-mar-2024

Google ScholarTM

Consultar

Altmetric

Altmetric


Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.