Please use this identifier to cite or link to this item: http://sgc.anlis.gob.ar/handle/123456789/352
Title: Detection of genetic polymorphism among and within Echinococcus granulosus strains by heteroduplex analysis of a microsatellite from the U1 snRNA genes
Authors: Roratto, P. A. 
Bartholomei-Santos, M. L. 
Gutierrez, Ariana M. 
Kamenetzky, Laura 
Rosenzvit, Mara C. 
Zaha, A. 
Keywords: Echinococcus granulosus;Repeticiones de Microsatélite;Ácidos Nucleicos Heterodúplex;Reacción en Cadena de la Polimerasa;Polimorfismo Genético
Issue Date: 2006
Description: Polymerase chain reaction of a pentanucleotide microsatellite in the U1 snRNA gene complex generated a multiple band pattern due to the priming of paralogous sequences. Denaturation and slow renaturation of polymerase chain reaction products allow the formation of heteroduplex DNA that can be detected by its differential mobility in polyacrylamide gel electrophoresis. Heteroduplex analysis was used to determine if the U1 snRNA microsatellite could be a useful genetic marker in Echinococcus granulosus. A U1 snRNA microsatellite fragment from E. granulosus was isolated and characterized by Southern blot and sequencing. Four E. granulosus strains were analyzed: sheep, Tasmanian sheep, cattle, and camel strains. The former two showed polymorphism and shared three of the six patterns found for sheep strain. The cattle strain displayed two patterns, and the camel strain was monomorphic. The electrophoretic profiles were used for statistical analysis in order to determine genetic distance and the relationship among strains. Heteroduplex analysis can be helpful in genotyping E. granulosus strains and is useful in detecting polymorphism within strains.
Fil: Roratto, P. A. Universidade Federal de Santa Maria. Departamento de Biologia; Brazil.
Fil: Bartholomei-Santos, M. L. Universidade Federal de Santa Maria. Departamento de Biologia; Brazil.
Fil: Gutierrez, Ariana M. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.
Fil: Kamenetzky, Laura. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.
Fil: Rosenzvit, Mara C. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.
Fil: Zaha, A. Universidade Federal do Rio Grande do Sul. 3Centro de Biotecnologia; Brazil.
URI: http://www.geneticsmr.com//year2006/vol5-3/pdf/gmr0187.pdf
http://sgc.anlis.gob.ar/handle/123456789/352
ISSN: 1676-5680
Rights: info:eu-repo/semantics/openAccess
Appears in Collections:Publicaciones INEI
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