Please use this identifier to cite or link to this item: http://sgc.anlis.gob.ar/handle/123456789/308
Title: Screening for active toxoplasmosis in patients by DNA hybridization with the ABGTg7 probe in blood samples.
Authors: Angel, Sergio O. 
Matrajt, Mariana 
Margarit, Juan 
Nigro, Monica 
Illescas, Edith 
Pszenny, Viviana 
Amendoeira, María Regina R. 
Guarnera, Eduardo 
Garberi, Juan C. 
Keywords: Toxoplasmosis;Toxoplasma;Hibridación Genética;Reacción en Cadena de la Polimerasa
Issue Date: 1997
Description: We report the potential use of a specific Toxoplasma gondii DNA probe (ABGTg7). We applied a dot blot hybridization assay to blood samples for the diagnosis of cerebral toxoplasmosis (CT), acute toxoplasmic lymphadenopathy (ATL), and disseminated toxoplasmosis in transplant recipients (TRs). We studied a total of 84 individuals: 38 patients and 46 controls. We found positive hybridization signals for 12 (66.7%) of 18 patients with confirmed CT, 9 (52.9%) of 17 patients with ATL, and 2 (66.7%) of 3 TRs. PCR assays were performed in parallel for patients with ATL, resulting in T. gondii DNA detection for 10 patients (58.8%). A comparative study between dot blot and PCR assays performed with the blood of mice that had been experimentally infected with tachyzoites gave similar results: 60 and 70% positive results, respectively. Finally, the sum of positive values obtained by both DNA tests (dot blot assay plus PCR) increased the rate of positivity for ATL patients to 76.4%. These results demonstrate that the T. gondii ABGTg7 repetitive DNA element is an additional useful resource for diagnosing Toxoplasma parasitemia in patients with CT and ATL and in TRs. Thus, our ABGTg7-based dot blot test may lead to an improvement in T. gondii detection methods in patients with acute toxoplasmosis.
Fil: Angel, Segio O. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Microbiología. Departamento Parasitología. Centro Nacional de Toxoplasmosis; Argentina.
Fil: Matrajt, Mariana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Microbiología. Departamento Parasitología. Centro Nacional de Toxoplasmosis; Argentina.
Fil: Margarit, Juan. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Microbiología. Departamento Parasitología. Centro Nacional de Toxoplasmosis; Argentina.
Fil: Nigro, Monica. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Microbiología. Departamento Parasitología. Centro Nacional de Toxoplasmosis; Argentina.
Fil: Illescas, Edith. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Microbiología. Departamento Parasitología. Centro Nacional de Toxoplasmosis; Argentina.
Fil: Pszenny, Viviana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Microbiología. Departamento Parasitología. Centro Nacional de Toxoplasmosis; Argentina.
Fil: Amendoeira, María Regina R. Instituto Oswaldo Cruz-Fiocruz. Departamento de Protozoología; Brazil.
Fil: Guarnera, Eduardo. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Microbiología. Departamento Parasitología. Centro Nacional de Toxoplasmosis; Argentina.
Fil: Garberi, Juan C. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Microbiología. Departamento Parasitología. Centro Nacional de Toxoplasmosis; Argentina.
URI: http://jcm.asm.org/content/35/3/591.full.pdf+html
http://sgc.anlis.gob.ar/handle/123456789/308
ISSN: 1098-660X
Rights: info:eu-repo/semantics/openAccess
Appears in Collections:Publicaciones INEI
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