Please use this identifier to cite or link to this item: http://sgc.anlis.gob.ar/handle/123456789/291
Title: Molecular identification of mumps virus genotypes from clinical samples: Standardized method of analysis
Authors: Palacios, Gustavo 
Jabado, Omar J. 
Cisterna, Daniel 
de Ory, F. 
Renwick, N. 
Echevarria, J. E. 
Castellanos, A. 
Mosquera, M. 
Freire, María Cecilia 
Campos, Rodolfo 
Lipkin, W. Ian 
Keywords: Virus de la Parotiditis;Sensibilidad y Especificidad;Reacción en Cadena de la Polimerasa
Issue Date: 2005
Description: A sensitive nested reverse transcription-PCR assay, targeting a short fragment of the gene encoding the small hydrophobic protein (SH gene), was developed to allow rapid characterization of mumps virus in clinical samples. The sensitivity and specificity of the assay were established using representative genotypes A, B, C, D, E, and F. Mumps virus RNA was characterized directly from cerebrospinal fluid (CSF) samples and in extracts of mumps virus isolates from patients with various clinical syndromes. Direct sequencing of products and subsequent phylogenetic analysis enabled genetic classification. A simple web-based system of sequence analysis was established. The study also allowed characterization of mumps virus strains from Argentina as part of a new subgenotype. This PCR assay for characterization of mumps infections coupled to a web-based analytical program provides a rapid method for identification of known and novel strains.
Fil: Palacios, Gustavo. Columbia University. Jerome L. and Dawn Greene Infectious Disease Laboratory; Estados Unidos.
Fil: Jabado, O. Columbia University. Jerome L. and Dawn Greene Infectious Disease Laboratory; Estados Unidos.
Fil: Cisterna, Daniel. Instituto de Salud Carlos III. Services of Diagnostic Microbiology and Virology; España.
Fil: de Ory, F. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología. División Neurovirosis; Argentina.
Fil: Renwick, N. Columbia University. Jerome L. and Dawn Greene Infectious Disease Laboratory; Estados Unidos.
Fil: Echevarria, J. E. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología. División Neurovirosis; Argentina.
Fil: Castellanos, A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología. División Neurovirosis; Argentina.
Fil: Mosquera, M. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología. División Neurovirosis; Argentina.
Fil: Freire, María Cecilia. tituto de Salud Carlos III. Services of Diagnostic Microbiology and Virology; España.
Fil: Campos, Rodolfo. Universidad de Buenos Aires. Cátedra de Virología; Argentina.
Fil: Lipkin, W. Ian. Columbia University. Jerome L. and Dawn Greene Infectious Disease Laboratory; Estados Unidos.
URI: http://jcm.asm.org/content/43/4/1869.full.pdf+html
http://sgc.anlis.gob.ar/handle/123456789/291
ISSN: 1098-660X
Rights: info:eu-repo/semantics/openAccess
Appears in Collections:Publicaciones INEI
snrd

Files in This Item:
File Description SizeFormat
JournalofClinicalMicrobiology,2005,43(4),1869–1878..pdfPDF715.96 kBAdobe PDFThumbnail
View/Open
ERRATUM.pdfPDF208.72 kBAdobe PDFThumbnail
View/Open
Show full item record

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.