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Title: | ROTADIAL: The first nanobody-based immunoassay to detect Group A Rotavirus | Authors: | Vega, C G Garaicoechea, L L Degiuseppe, J I Bok, M Rivolta, A A Piantanida, A P Asenzo, G Adúriz Guerrero, M Wigdorovitz, A Stupka, J A Parreño, V |
Keywords: | Infecciones por Rotavirus;Análisis de Regresión;Ensayo de Inmunoadsorción Enzimática | Issue Date: | Dec-2021 | Journal: | Journal of virological methods | Abstract: | ROTADIAL is a rapid nanobody (Nb)-based ELISA assay able to identify Rotavirus group A (RVA) in feces from pediatric patients. The assay is based on a sandwich of two patented llama-derived Nbs directed to the inner capsid viral protein VP6 from RVA. Nbs are directed to conformational epitopes of VP6 and recognized all human RVA strains tested, representing ideal reagents for their use in immunodiagnostic tests for RVA detection. All the steps are carried out at room temperature, bringing results in less than two hours. This assay, named ROTADIAL, was validated with a reference panel of feces from pediatric patients from Argentina. The overall sensitivity and specificity of the ROTADIAL test, when compared to a commercial test, was 100 % (100/100) and 99 % (99/100) respectively. ROTADIAL presented optimal analytical performance, being capable of detecting RVA regardless of the presence of other common human enteric infectious agents and is the first RVA-diagnostic assay developed using Nbs, worldwide. |
Description: | Fil. Vega, CG. Incuinta, Instituto Nacional de Investigaciones Agropecuarias (INTA), Argentina; IVIT, CONICET, Argentina. Fil. Garaicoechea, LL. Incuinta, Instituto Nacional de Investigaciones Agropecuarias (INTA), Argentina. Fil. Degiuseppe, JI. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología; Argentina. Fil. Bok, M. Incuinta, Instituto Nacional de Investigaciones Agropecuarias (INTA), Argentina; IVIT, CONICET, Argentina. Fil. Rivolta, AA. Incuinta, Instituto Nacional de Investigaciones Agropecuarias (INTA), Argentina. Fil. Piantanida, AP. Incuinta, Instituto Nacional de Investigaciones Agropecuarias (INTA), Argentina. Fil. Asenzo, G. Incuinta, Instituto Nacional de Investigaciones Agropecuarias (INTA), Argentina. IVIT, CONICET, Argentina. Fil. Adúriz Guerrero, M. Incuinta, Instituto Nacional de Investigaciones Agropecuarias (INTA), Argentina. IVIT, CONICET, Argentina. Fil. Wigdorovitz, Un. Incuinta, Instituto Nacional de Investigaciones Agropecuarias (INTA), Argentina. IVIT, CONICET, Argentina. Fil. Stupka, JA. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología; Argentina. Fil. Parreño, V. Incuinta, Instituto Nacional de Investigaciones Agropecuarias (INTA), Argentina. IVIT, CONICET, Argentina. |
URI: | http://sgc.anlis.gob.ar/handle/123456789/2405 | DOI: | 10.1016/j.jviromet.2021.114279 |
Appears in Collections: | Publicaciones INEI |
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1-s2.0-S0166093421002184-main inei 2 5-11.pdf | Artículo en inglés | 2.56 MB | Adobe PDF | View/Open |
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