Please use this identifier to cite or link to this item: http://sgc.anlis.gob.ar/handle/123456789/2383
Title: Application of quantitative immunofluorescence assays to analyze the expression of cell contact proteins during Zika virus infections
Authors: Leiva, Santiago 
Dizanzo, María Paula 
Fabbri, Cintia 
Bugnon Valdano, Marina 
Luppo, Victoria 
Levis, Silvana 
Cavatorta, Ana Laura 
Morales, María Alejandra 
Gardiol, Daniela 
Keywords: Carácter Cuantitativo Heredable;Adhesiones Focales;Ocludina;Enfermedad del Virus Zika
Issue Date: 15-Oct-2021
Journal: Virus research 
Abstract: 
Zika Virus (ZIKV) is an RNA virus that belongs to the Flavivirus (FV) genus. In the last years, several unique characteristics of ZIKV among FV have been revealed, as the multiple routes of transmission and its ability to reach different human tissues, including the central nervous system. Thus, one of the most intriguing features of ZIKV biology is its ability to cross diverse complex biological barriers. The main aim of this study is to contribute to the understanding of the still unclear mechanisms behind this viral activity. We investigated an African strain and two South American ZIKV isolates belonging to the Asian lineage, in order to characterize possible differences regarding their ability to disturb intercellular junctions. The Asian isolates correspond to an imported (Venezuelan) and an autochthonous (Argentinian) ZIKV strain for which there is still no data available. We focused on occludin and DLG1 expression as markers of tight and adherent junctions, respectively. For this, we applied a quantitative immunofluorescence assay that can ascertain alterations in the cell junction proteins expression in the infected cells. Our findings indicated that the different ZIKV strains were able to reduce the levels of both polarity proteins without altering their overall cell distribution. Moreover, the grade of this effect was strain-dependent, being the DLG1 reduction higher for the African and Asian Venezuelan isolates and, on the contrary, occludin down-regulation was more noticeable for the Argentinian strain. Interestingly, among both junction proteins the viral infection caused a relative larger reduction in DLG1 expression for all viruses, suggesting DLG1 may be of particular relevance for ZIKV infections. Taken together, this study contributes to the knowledge of the biological mechanisms involved in ZIKV cytopathogenesis, with a special focus on regional isolates.
Description: 
Fil. Leiva, Santiago. Instituto de Biología Molecular y Celular de Rosario-CONICET, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario. Rosario, Argentina.

Fil. Dizanzo, María Paula. Instituto de Biología Molecular y Celular de Rosario-CONICET, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario. Rosario, Argentina.

Fil. Cintia Fabbri. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Virales Humanas; Argentina.

Fil. Bugnon Valdano, Marina. Instituto de Biología Molecular y Celular de Rosario-CONICET, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario. Rosario, Argentina.

Fil. Luppo, Victoria. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Virales Humanas; Argentina.

Fil. Levis, Silvana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Virales Humanas; Argentina.

Fil. Cavatorta, Ana Laura. Instituto de Biología Molecular y Celular de Rosario-CONICET, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario. Rosario, Argentina.

Fil. Morales, María Alejandra. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Virales Humanas; Argentina.

Fil. Gardiol, Daniela. Instituto de Biología Molecular y Celular de Rosario-CONICET, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario. Rosario, Argentina.
URI: http://sgc.anlis.gob.ar/handle/123456789/2383
DOI: 10.1016/j.virusres.2021.198544
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