Please use this identifier to cite or link to this item: http://sgc.anlis.gob.ar/handle/123456789/220
Title: Susceptibility Patterns and Molecular Identification of Trichosporon Species
Authors: Rodríguez-Tudela, Juan L. 
Diaz-Guerra, Teresa M. 
Mellado, Emilia 
Cano, Virginia 
Tapia, Cecilia 
Perkins, Alexander 
Gomez-Lopez, Alicia 
Rodero, Laura 
Cuenca-Estrella, Manuel 
Keywords: Trichosporon;Farmacorresistencia Fúngica
Issue Date: Oct-2005
Publisher: American Society for Microbiology
Journal: Antimicrobial agents and chemotherapy 
Abstract: 
The physiological patterns, the sequence polymorphisms of the internal transcriber spacer (ITS), and intergenic spacer regions (IGS) of the rRNA genes and the antifungal susceptibility profile were evaluated for their ability to identify Trichosporon spp. and their specificity for the identification of 49 clinical isolates of Trichosporon spp. Morphological and biochemical methodologies were unable to differentiate among the Trichosporon species. ITS sequencing was also unable to differentiate several species. However, IGS1 sequencing unambiguously identified all Trichosporon isolates. Following the results of DNA-based identification, Trichosporon asahii was the species most frequently isolated from deep sites (15 of 25 strains; 60%). In the main, other Trichosporon species were recovered from cutaneous samples. The majority of T. asahii, T. faecale, and T. coremiiforme clinical isolates exhibited resistance in vitro to amphotericin B, with geometric mean (GM) MICs >4 mug/ml. The other species of Trichosporon did not show high MICs of amphotericin B, and GM MICs were <1 mug/ml. Azole agents were active in vitro against the majority of clinical strains. The most potent compound in vitro was voriconazole, with a GM MIC
Description: 
Fil: Rodriguez-Tudela, Juan L. Instituto de Salud Carlos III. Servicio de Micología; España.

Fil: Diaz-Guerra, Teresa M. Instituto de Salud Carlos III. Servicio de Micología; España.

Fil: Mellado, Emilia. Instituto de Salud Carlos III. Servicio de Micología; España.

Fil: Cano, Virginia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.

Fil: Tapia, Cecilia. Universidad de Chile. Programa de Microbiología y Micología; Chile.

Fil: Perkins, Alexander. Instituto de Salud Carlos III. Servicio de Micología; España.

Fil: Gomez-Lopez, Alicia. Instituto de Salud Carlos III. Servicio de Micología; España.

Fil: Rodero, Laura. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.

Fil: Cuenca-Estrella, Manuel. Instituto de Salud Carlos III. Servicio de Micología; España.
URI: http://sgc.anlis.gob.ar/handle/123456789/220
http://aac.asm.org/content/49/10/4026.full.pdf+html
ISSN: 1098-6596
DOI: 10.1128-AAC.49.10.4026-4034.2005 Free PMC article
Rights: info:eu-repo/semantics/openAccess
Open Access
Creative Commons Attribution 4.0 International License
Appears in Collections:snrd
Publicaciones INEI

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