Please use this identifier to cite or link to this item:
http://sgc.anlis.gob.ar/handle/123456789/2145
DC Field | Value | Language |
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dc.contributor.author | Nigro, Mónica G | es |
dc.contributor.author | Martín, Valentina | es |
dc.contributor.author | Kaufer, Federico | es |
dc.contributor.author | Carral, Liliana | es |
dc.contributor.author | Angel, Sergio O. | es |
dc.contributor.author | Pszenny, Viviana | es |
dc.date.accessioned | 2021-01-15T16:06:00Z | - |
dc.date.available | 2021-01-15T16:06:00Z | - |
dc.date.issued | 2001-07 | - |
dc.identifier.issn | 1073-6085 | - |
dc.identifier.uri | http://sgc.anlis.gob.ar/handle/123456789/2145 | - |
dc.description | Fil: Nigro, Mónica. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología. Departamento de Parasitología Sanitaria; Argentina. | es |
dc.description | Fil: Martin, Valentina. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología. Departamento de Parasitología Sanitaria; Argentina. | es |
dc.description | Fil: Kaufer, Federico. Hospital Alemán. Laboratorio de Toxoplasmosis; Argentina. | es |
dc.description | Fil: Carral, Liliana. Hospital Alemán. Laboratorio de Toxoplasmosis; Argentina. | es |
dc.description | Fil: Angel, Sergio O. Instituto Nacional de Parasitología. Departamento de Parasitología Sanitaria; Argentina. | es |
dc.description | Fil: Pszenny, Viviana. Instituto Nacional de Parasitología. Departamento de Parasitología Sanitaria; Argentina. | es |
dc.description.abstract | The rhoptry 2 protein (Rop2) is an interesting protein of Toxoplasma gondii that is involved in the parasite invasion of host cell, it has three T-cell epitopes and high antigenic value. However, the expression of Rop2 as a recombinant protein in Escherichia coli is not an easy task, showing low levels of expression or degradation and solubility problems. Using a recombinant Rop2(196-561) fused to 6 histidine residues, we showed high levels of expression in bacteria growing in terrific broth. rRop2(196-561) was purified mainly as a soluble product and in high concentrations (approx 1 mg/mL) under native conditions (40 mM imidazol in phosphate buffer). However, after a cycle of freezing-thawing rRop2(196-561) became insoluble. When glycerol was added to 26%, immediately after purification, the protein stayed soluble after cycles of freezing-thawing. Finally, it was demonstrated that under these conditions soluble rRop2(196-561) keeps its diagnostic value in contrast with the insoluble protein. | es |
dc.language.iso | en | es |
dc.publisher | Springer | es |
dc.relation | datasets | - |
dc.relation.ispartof | Molecular biotechnology | es |
dc.rights | Open Access | - |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | - |
dc.source | Molecular Biotechnology 2001; 18(3):269-273 | - |
dc.subject | Animales | es |
dc.subject | Antígenos de Protozoos | es |
dc.subject | Humanos | es |
dc.subject | Proteínas de la Membrana | es |
dc.subject | Proteínas Protozoarias | es |
dc.subject | Proteínas Recombinantes de Fusión | es |
dc.subject | Solubilidad | es |
dc.subject | Toxoplasma | es |
dc.subject | Toxoplasmosis | es |
dc.subject | Escherichia coli | es |
dc.subject | Expresión Génica | es |
dc.subject | Vectores Genéticos | es |
dc.title | High level of expression of the Toxoplasma gondii-recombinant Rop2 protein in Escherichia coli as a soluble form for optimal use in diagnosis | es |
dc.type | Artículo | es |
dc.rights.license | Creative Commons Attribution 4.0 International License | - |
dc.identifier.doi | 10.1385/MB:18:3:269 | - |
anlis.essnrd | 1 | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.openairetype | Artículo | - |
item.fulltext | No Fulltext | - |
item.languageiso639-1 | en | - |
Appears in Collections: | Publicaciones INP |
This item is licensed under a Creative Commons License