Please use this identifier to cite or link to this item: http://sgc.anlis.gob.ar/handle/123456789/2129
Title: Pilot field trial of the EG95 vaccine against ovine cystic echinococcosis in Rio Negro, Argentina: 8 years of work
Authors: Larrieu, Edmundo 
Mujica, Guillermo 
Araya, Daniel 
Labanchi, Jose Luis 
Arezo, Marcos 
Herrero, Eduardo 
Santillan, Graciela 
Vizcaychipi, Katherina A. 
Uchiumi, Leonardo 
Salvitti, Juan Carlos 
Grizmado, Claudia 
Calabro, Arnoldo 
Talmon, Gabriel 
Sepulveda, Luis 
Galvan, Jose Maria 
Cabrera, Marta 
Seleiman, Marcos 
Crowley, Pablo 
Céspedes, Graciela 
García Cachau, Mariela 
Gino, Lilia 
Molina, Leonardo 
Daffner, Jose 
Gauci, Charles G 
Donadeu, Meritxell 
Lightowlers, Marshall W 
Keywords: Control;Equinococosis;Diagnóstico;Ovinos;Vacunación
Issue Date: Mar-2019
Journal: Acta tropica 
Abstract: 
Cystic echinococcosis (CE) is endemic in the Rio Negro province of Argentina. After 30 years of control using praziquantel in dogs the transmission rate to humans and sheep has decreased significantly, however transmission persists. The objective of the study was to assess the inclusion of the EG95 for sheep in the control program and to determine the vaccine's operative feasibility in field conditions. An intervention study was defined in Rio Negro Province in Argentina comprising, in total, an area of 5820 Km2. Lambs received two vaccinations with the EG95 vaccine followed by a single booster injection when the animals were 1-1.5 years of age. Vaccination of lambs born into one trial site was introduced and continued for 8 years. Evidence for Echinococcus granulosus transmission was monitored before and after vaccination by coproantigen ELISA in faecal samples of dog, purgation of dogs to detect E. granulosus worms, necropsy on adult sheep and by ultrasound screening in children of 6-14 years old. 29,323 doses of vaccine were applied between 2009 and 2017, which a vaccination coverage of 80.1%/85.7% (57.3% average for fully vaccinated). Before the introduction of the vaccine 56.3% of the 6-year-old sheep were infected with E. granulosus at necropsy and 84.2% of the farms had infected sheep; 4.3% of the dogs were positive for E. granulosus infection using the arecoline test, and with coproELISA 9.6% of dog fecal samples were positive and 20.3% of the farms had infected dog.After the vaccine was introduced, 21.6% of sheep older than 6 years were found to be infected at necropsy and 20.2% of the farms were found to be infected; in dogs, 4.5% were found positive for E. granulosus using arecoline purgation and with coproELISA 3.7% of samples were positive, with 8.9% of farms having a positive dog. In 2016 only one case of E. granulosus infection was diagnosed by US screening in a 6-14 years old child. Included in the analysis are discussions of difficulties experienced in the field which affected correct vaccine administration as well as social features and practices that may impact on echinococcosis control and the EG95 vaccination program in Rio Negro. Vaccination of sheep with the EG95 vaccine provides a valuable new tool which improves the effectiveness of CE control activities. Vaccination was effective even in a difficult, remote environment where only approximately half the lambs born into the communities were fully vaccinated.
Description: 
Fil: Larrieu, Edmundo. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina.

Fil: Mujica, Guillermo. Ministerio de Salud, Provincia de Río Negro; Argentina.

Fil: Araya, Daniel. Ministerio de Salud, Provincia de Río Negro; Argentina.

Fil: Labanchi, Jose Luis. Ministerio de Salud, Provincia de Río Negro; Argentina.

Fil: Arezo, Marcos. Ministerio de Salud, Provincia de Río Negro; Argentina.

Fil: Herrero, Eduardo. Ministerio de Salud, Provincia de Río Negro; Argentina.

Fil: Santillán, Graciela. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.

Fil: Vizcaychipi, Katherina A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.

Fil: Uchiumi, Leonardo. Ministerio de Salud, Provincia de Río Negro; Argentina.

Fil: Salvitti, Juan Carlos. Ministerio de Salud, Provincia de Río Negro; Argentina.

Fil: Grizmado, Claudia. Ministerio de Salud, Provincia de Río Negro; Argentina.

Fil: Calabro, Arnoldo. Ministerio de Salud, Provincia de Río Negro; Argentina.

Fil: Talmon, Gabriel. Ministerio de Salud, Provincia de Río Negro; Argentina.

Fil: Sepulveda, Luis. Ministerio de Salud, Provincia de Río Negro; Argentina.

Fil: Galvan, Jose María. Ministerio de Salud, Provincia de Río Negro; Argentina.

Fil: Cabrera, Marta. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.

Fil: Seleiman, Marcos. Ministerio de Salud, Provincia de Río Negro; Argentina.

Fil: Crowley, Pablo. Universidad Nacional de Río Negro. Escuela de Veterinaria; Argentina.

Fil: Cespedes, Graciela. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.

Fil: García Cachau, Mariela. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina.

Fil: Gino, Lilia. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina.

Fil: Molina, Leonardo. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina.

Fil: Daffner, Jose. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.

Fil: Gauci, Charles G. University of Melbourne. Faculty of Veterinary and Agricultural Sciences; Australia.

Fil: Donadeu, Meritxell. University of Melbourne. Faculty of Veterinary and Agricultural Sciences; Australia.

Fil: Lightowlers, Marshall W. University of Melbourne. Faculty of Veterinary and Agricultural Sciences; Australia.
URI: http://sgc.anlis.gob.ar/handle/123456789/2129
ISSN: 0001-706X
DOI: 10.1016/j.actatropica.2018.12.025
Rights: Closed Access
Appears in Collections:Publicaciones INEI

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