Use este identificador para citar ou linkar para este item:
http://sgc.anlis.gob.ar/handle/123456789/2092
Título: | Shiga toxin-producing Escherichia coli O157 : H7 shows an increased pathogenicity in mice after the passage through the gastrointestinal tract of the same host | Autor(es): | Fernandez-Brando, Romina J Miliwebsky, Elizabeth Mejías, M P Baschkier, Ariela Panek, C A Abrey-Recalde, M J Cabrera, Gabriel Ramos, María Victoria Rivas, Marta Palermo, M. |
Palavras-chave: | Toxina Shiga;Escherichia coli;Trombocitopenia | Data do documento: | Jun-2012 | Editora: | Microbiology Society | Jornal: | Journal of medical microbiology | Resumo: | Haemolytic uraemic syndrome (HUS) is a rare but life-threatening complication of Shiga toxin (Stx)-producing Escherichia coli (STEC) infections, characterized by acute renal failure, thrombocytopenia and haemolytic anaemia. Although the main infection route is the consumption of contaminated food or water, person-to-person transmission has been suggested in several situations. Moreover, epidemiological data indicate that the horizontal transmission of several pathogens, including STEC, among individuals of the same species requires significantly lower doses than those used in animal models infected with laboratory-cultured bacteria. Thus, the aim of this study was to evaluate whether the passage of a clinically isolated STEC strain through the gastrointestinal tract of mice affects its pathogenicity in mice. To test this, weaned mice were orally inoculated by gavage with either an E. coli O157:H7 isolate from an HUS patient, or the same strain recovered from stools after one or two successive passages through the gastrointestinal tract of the mice. We show that stool-recovered strains are able to induce a more generalized and persistent colonization than the parent strain. Furthermore, a 10(4)-fold-reduced inoculum of the stool-recovered strains still causes gut colonization and mouse mortality, which are not observed with the parent strain. These results indicate an increased pathogenicity in stool-recovered strains that may be associated with an increased ability to colonize the mouse intestine. |
Descrição: | Fil: Fernandez-Brando, Romina J. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina. Fil: Miliwebsky, Elizabeth. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina. Fil: Mejías, M P. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina. Fil: Baschkier, Ariela. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina. Fil: Panek, C A. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina. Fil: Abrey-Recalde, M J. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina. Fil: Cabrera, Gabriel. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina. Fil: Ramos, María Victoria. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina. Fil: Rivas, Marta. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina. Fil: Palermo, M. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina. |
URI: | https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.041251-0#tab2 http://sgc.anlis.gob.ar/handle/123456789/2092 |
ISSN: | 1473-5644 | DOI: | 10.1099/jmm.0.041251-0 | Direitos: | Open Access Creative Commons Attribution 4.0 International License |
Aparece nas Coleções: | Publicaciones INEI |
Arquivos neste item:
Arquivo | Descrição | Tamanho | Formato | |
---|---|---|---|---|
10.1099_jmm.0.041251-0.pdf | Artículo en inglés | 377.28 kB | Adobe PDF | Ver/Aberto |
Visualização de página
37
Checado em 15/10/2024
Download(s)
8
Checado em 15/10/2024
Google ScholarTM
Checar
Altmetric
Altmetric
Este item está licenciada sob uma Licença Creative Commons