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Title: | Indirect immunofluorescence assay for the simultaneous detection of antibodies against clinically important old and new world hantaviruses | Authors: | Lederer, Sabine Lattwein, Erik Hanke, Merle Sonnenberg, Karen Stoecker, Winfried Lundkvist, Åke Vaheri, Antti Vapalahti, Olli Chan, Paul K. S. Feldmann, Heinz Dick, Daryl Schmidt-Chanasit, Jonas Padula, Paula Vial, Pablo A Panculescu-Gatej, Raluca Ceianu, Cornelia Heyman, Paul Avšič-Županc, Tatjana Niedrig, Matthias |
Keywords: | Anticuerpos Antivirales;Técnica del Anticuerpo Fluorescente Indirecta;Hantavirus;Infecciones por Hantavirus;Microscopía Fluorescente | Issue Date: | 4-Apr-2013 | Journal: | PLoS neglected tropical diseases | Abstract: | In order to detect serum antibodies against clinically important Old and New World hantaviruses simultaneously, multiparametric indirect immunofluorescence assays (IFAs) based on biochip mosaics were developed. Each of the mosaic substrates consisted of cells infected with one of the virus types Hantaan (HTNV), Puumala (PUUV), Seoul (SEOV), Saaremaa (SAAV), Dobrava (DOBV), Sin Nombre (SNV) or Andes (ANDV). For assay evaluation, serum IgG and IgM antibodies were analyzed using 184 laboratory-confirmed hantavirus-positive sera collected at six diagnostic centers from patients actively or previously infected with the following hantavirus serotypes: PUUV (Finland, n=97); SEOV (China, n=5); DOBV (Romania, n=7); SNV (Canada, n=23); ANDV (Argentina and Chile, n=52). The control panel comprised 89 sera from healthy blood donors. According to the reference tests, all 184 patient samples were seropositive for hantavirus-specific IgG (n=177; 96%) and/or IgM (n=131; 72%), while all control samples were tested negative. In the multiparametric IFA applied in this study, 183 (99%) of the patient sera were IgG and 131 (71%) IgM positive (accordance with the reference tests: IgG, 96%; IgM, 93%). Overall IFA sensitivity for combined IgG and IgM analysis amounted to 100% for all serotypes, except for SNV (96%). Of the 89 control sera, 2 (2%) showed IgG reactivity against the HTNV substrate, but not against any other hantavirus. Due to the high cross-reactivity of hantaviral nucleocapsid proteins, endpoint titrations were conducted, allowing serotype determination in >90% of PUUV- and ANDV-infected patients. Thus, multiparametric IFA enables highly sensitive and specific serological diagnosis of hantavirus infections and can be used to differentiate PUUV and ANDV infection from infections with Murinae-borne hantaviruses (e.g. DOBV and SEOV). |
Description: | Fil: Lederer, Sabine. EUROIMMUN Medizinische Labordiagnostika AG; Alemania. Fil: Lattwein, Erik. EUROIMMUN Medizinische Labordiagnostika AG; Alemania. Fil: Hanke, Merle. EUROIMMUN Medizinische Labordiagnostika AG; Alemania. Fil: Sonnenberg, Karen. EUROIMMUN Medizinische Labordiagnostika AG; Alemania. Fil: Stoecker, Winfried. EUROIMMUN Medizinische Labordiagnostika AG; Alemania. Fil: Lundkvist, Åke. Karolinska Institute. Swedish Institute for Infectious Disease Control; Suecia. Fil: Vaheri, Antti. University of Helsinki. Haartman Institute. Department of Virology; Finlandia. Fil: Vapalahti, Olli. University of Helsinki. Haartman Institute. Department of Virology; Finlandia. Fil: Chan, Paul K. S. Chinese University of Hong Kong. Faculty of Medicine. Department of Microbiology, Hong Kong; República Popular China. Fil: Feldmann, Heinz. Canadian Science Centre for Human and Animal Health. Public Health Agency of Canada. National Microbiology Laboratory. Zoonotic Diseases and Special Pathogens. Special Pathogens Program; Canadá. Fil: Dick, Daryl. Canadian Science Centre for Human and Animal Health. Public Health Agency of Canada. National Microbiology Laboratory. Zoonotic Diseases and Special Pathogens. Special Pathogens Program; Canadá. Fil: Schmidt-Chanasit, Jonas. Bernhard Nocht Institute for Tropical Medicine. WHO Collaborating Centre for Arbovirus and Haemorrhagic Fever Reference and Research; Alemania. Fil: Padula, Paula. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología; Argentina. Fil: Vial, Pablo A. Universidad del Desarrollo. Institute of Science. Clínica Alemana School of Medicine; Chile. Fil: Panculescu-Gatej, Raluca. CANTACUZINO National Institute for Research and Development in Microbiology and Immunology. Laboratory of Vector-Borne Infections and Medical Entomology; Rumanía. Fil: Ceianu, Cornelia. CANTACUZINO National Institute for Research and Development in Microbiology and Immunology. Laboratory of Vector-Borne Infections and Medical Entomology; Rumanía. Fil: Heyman, Paul. Queen Astrid Military Hospital. Research Laboratory for Vector-Borne Diseases; Bélgica. Fil: Avšič-Županc, Tatjana. Institute of Microbiology and Immunology. Laboratory for Diagnosis of Zoonoses and WHO Laboratory; Eslovenia. Fil: Niedrig, Matthias. Robert Koch Institute. Center for Biological Safety (ZBS-1); Alemania. |
URI: | http://sgc.anlis.gob.ar/handle/123456789/1928 | ISSN: | 1935-2735 | DOI: | 10.1371/journal.pntd.0002157 | Rights: | Open Access Creative Commons Attribution 4.0 International License |
Appears in Collections: | Publicaciones INEI Publicaciones INEI |
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