Please use this identifier to cite or link to this item: http://sgc.anlis.gob.ar/handle/123456789/1892
Title: Evaluation of an enzyme-linked immunosorbent assay for detection of antibodies to Junin virus in rodents
Authors: Morales, María Alejandra 
Calderón, Gladys 
Riera, Laura 
Ambrosio, Ana María 
Enria, Delia 
Sabattini, Marta S. 
Keywords: Animales;Animales Salvajes;Anticuerpos Antivirales;Infecciones por Arenaviridae;Chlorocebus aethiops;Reservorios de Enfermedades;Técnica del Anticuerpo Fluorescente Indirecta;Inmunoglobulina G;Virus Junin;Muridae;Reproducibilidad de los Resultados;Enfermedades de los Roedores;Sensibilidad y Especificidad;Células Vero;Ensayo de Inmunoadsorción Enzimática
Issue Date: May-2002
Publisher: Elsevier
Project: datasets
Journal: Journal of virological methods 
Abstract: 
Junin virus is the etiological agent of Argentine hemorrhagic fever, a serious rodent-borne disease. An enzyme-linked immunosorbent assay (ELISA) to detect Junin virus IgG antibodies in rodents was evaluated using sera from 27 Calomys musculinus and five Calomys laucha, inoculated experimentally with a live attenuated strain of this arenavirus. The test performance was compared against an indirect immunofluorescence assay (IFA). The ELISA had a sensitivity and specificity of 100% and a reproducibility of 87.9% for samples with titers above the selected cut-off value. IFA had lower sensitivity (53%) with the same specificity. The ELISA results were similar, whether carried out on whole blood or serum samples, thus eliminating the need for serum separation. A high correlation (K=0.86) between ELISA and IFA results was obtained from 1011 wild sigmodontine and murine rodents collected within and outside of the Argentine hemorrhagic fever endemic area. These results indicate that Junin virus IgG ELISA is the most suitable assay for detection of Junin virus antibodies in rodent samples.
Description: 
Fil: Morales, María A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Virales Humanas; Argentina.

Fil: Calderón, Gladys E. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Virales Humanas; Argentina.

Fil: Riera, Laura M. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Virales Humanas; Argentina.

Fil: Ambrosio, Ana M. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Virales Humanas; Argentina.

Fil: Enría, Delia A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Virales Humanas; Argentina.

Fil: Sabattini, Marta S. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Virales Humanas; Argentina.
URI: http://sgc.anlis.gob.ar/handle/123456789/1892
ISSN: 0166-0934
DOI: 10.1016/s0166-0934(01)00452-9
Rights: Open Access
Appears in Collections:Publicaciones INEVH

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